Comparative profiling and comprehensive quantification of stratum corneum ceramides in humans and mice by LC/MS/MS[S]
Autor: | Momoko Kawana, Yusuke Ohno, Akio Kihara, Masatoshi Miyamoto |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Skin barrier Ceramide QD415-436 030204 cardiovascular system & hematology Ceramides Biochemistry Pathogenesis Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endocrinology Tandem Mass Spectrometry epidermis Lipidomics Stratum corneum medicine Animals Humans skin barrier Research Articles Barrier function mass spectrometry chemistry.chemical_classification sphingolipids Fatty acid Cell Biology Sphingolipid 030104 developmental biology medicine.anatomical_structure chemistry lipidomics fatty acid Chromatography Liquid |
Zdroj: | Journal of Lipid Research, Vol 61, Iss 6, Pp 884-895 (2020) J Lipid Res |
ISSN: | 0022-2275 |
Popis: | Ceramides are the predominant lipids in the stratum corneum (SC) and are crucial components for normal skin barrier function. Although the composition of various ceramide classes in the human SC has been reported, that in mice is still unknown, despite mice being widely used as animal models of skin barrier function. Here, we performed LC/MS/MS analyses using recently available ceramide class standards to measure 25 classes of free ceramides and 5 classes of protein-bound ceramides from human and mouse SC. Phytosphingosine- and 6-hydroxy sphingosine-type ceramides, which both contain an additional hydroxyl group, were abundant in the human SC (35% and 45% of total ceramides, respectively). In contrast, in mice, phytosph-ingosine- and 6-hydroxy sphingosine-type ceramides were present at similar to 1% and undetectable levels, respectively, and sphingosine-type ceramides accounted for similar to 90%. In humans, ceramides containing alpha-hydroxy FA were abundant, whereas ceramides containing beta-hydroxy or omega-hydroxy FA were abundant in mice. The hydroxylated beta-carbon in beta-hydroxy ceramides was in the (R) configuration. Genetic knockout of beta-hydroxy acyl-CoA dehydratases in HAP1 cells increased beta-hydroxy ceramide levels, suggesting that beta-hydroxy acyl-CoA, an FA-elongation cycle intermediate in the ER, is a substrate for beta-hydroxy ceramide synthesis. We anticipate that our methods and findings will help to elucidate the role of each ceramide class in skin barrier formation and in the pathogenesis of skin disorders. |
Databáze: | OpenAIRE |
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