Dedifferentiated fat cells in polyglycolic acid-collagen nerve conduits promote rat facial nerve regeneration
| Autor: | Kazuki Hashimoto, Yoshifumi Ueta, Tomohiko Kazama, Yosuke Niimi, Mariko Miyata, Hironobu Osaki, Wataru Kamei, Hajime Matsumine, Hiroyuki Sakurai, Taro Matsumoto, Kaori Fujii, Mari Shimizu, Hiroshi Fujimaki |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty DFAT cells Biomedical Engineering Nerve guidance conduit Adipose tissue Article Biomaterials 03 medical and health sciences Myelin 0302 clinical medicine PGA polyglycolic acid medicine SVF stromal vascular fraction lcsh:QH573-671 PO propylene oxide lcsh:R5-920 Stem cell Chemistry lcsh:Cytology Skeletal muscle Facial nerve VEGF vascular endothelial growth factor Compound muscle action potential Nerve regeneration 030104 developmental biology medicine.anatomical_structure ADSC adipose-derived stem cell DFAT dedifferentiated fat Original Article lcsh:Medicine (General) 030217 neurology & neurosurgery Type I collagen Developmental Biology Reinnervation |
| Zdroj: | Regenerative Therapy, Vol 11, Iss, Pp 240-248 (2019) Regen Ther Regenerative Therapy |
| ISSN: | 2352-3204 |
| Popis: | Introduction Polyglycolic acid (PGA) nerve conduits, an artificial biodegradable nerve regeneration-inducing tube currently used in clinical practice, are effective in regenerating peripheral nerves. Dedifferentiated fat (DFAT) cells differentiate into various cells including adipocytes, osteoblasts, chondrocytes, skeletal muscle cells, and myofibroblasts, when cultured in appropriate differentiation-inducing conditioned culture medium. This study made a hybrid artificial nerve conduit by filling a PGA conduit with DFAT cells, applied the conduit to a rat facial nerve defect model, and investigated the facial nerve regenerative ability of the conduit. Methods Under inhalational anesthesia, the buccal branch of the facial nerve in Lewis rats was exposed, and a 7-mm nerve defect was created. PGA nerve conduits were filled with DFAT cells, which were prepared from rat subcutaneous adipose tissue with type I collagen as a scaffold, and then grafted into the nerve defect sites in rats with a microscope (DFAT group) (n = 10). In other rats, PGA artificial nerve conduits alone were similarly grafted into the nerve defect sites (the control group) (n = 10). Reinnervation was confirmed at 13 weeks postoperatively by a retrograde tracer, followed by histological and physiological comparative studies. Results The mean number of myelinated fibers was significantly higher in DFAT group (1605 ± 806.23) than in the control group (543.6 ± 478.66). Myelin thickness was also significantly lager in DFAT group (0.57 ± 0.17 μm) than in the control group. (0.46 ± 0.14 μm). Although no significant difference was found in the amplitude of compound muscle action potential (CMAP) between DFAT group (2.84 ± 2.47 mV) and the control group (0.88 ± 0.56 mV), whisker motion was lager in DFAT group (9.22° ± 0.65°) than in the control group (1.9° ± 0.84°). Conclusions DFAT cell-filled PGA conduits were found to promote nerve regeneration in an experimental rat facial nerve defect model. Highlights • PGA artificial conduits containing DFAT cells were made in this study. • The facial nerve regenerative ability of conduits was evaluated in a rat model. • Reinnervation was confirmed at 13 weeks postoperatively. • The nerve regeneration promoting effect of DFAT cells was found in the model. |
| Databáze: | OpenAIRE |
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