Trichinella spiralis: impact on the expression of Toll-like receptor 4 (TLR4) gene during the intestinal phase of experimental trichinellosis
| Autor: | Elżbieta Wandurska-Nowak, Wiesława Jankowska, Edward Hadaś, Monika Derda, Paweł P. Jagodziński, Agnieszka Wojtkowiak-Giera |
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| Rok vydání: | 2018 |
| Předmět: |
trichinellosis
0301 basic medicine Veterinary medicine toll-like receptor 4 q-PCR Trichinella spiralis Biology Microbiology Jejunum 03 medical and health sciences SF600-1100 medicine Receptor mouse Toll-like receptor intestines Innate immune system General Veterinary biology.organism_classification Small intestine 030104 developmental biology Real-time polymerase chain reaction medicine.anatomical_structure immunohistochemical staining TLR4 Research Article |
| Zdroj: | Journal of Veterinary Research Journal of Veterinary Research, Vol 62, Iss 4, Pp 493-496 (2018) |
| ISSN: | 2450-8608 |
| DOI: | 10.2478/jvetres-2018-0063 |
| Popis: | Introduction: Toll-like receptors (TLRs) play a key role in the rapid activation of the innate immune response to a variety of pathogens. The aim of this study was to evaluate the effect of Trichinella spiralis infection on the level of expression of the tlr4 gene in mouse intestines during the intestinal phase of experimental trichinellosis. Material and Methods: The experimental material consisted of the small and large intestines of BALB/c mice infected with Trichinella spiralis sampled at 4, 8, and 16 days post infection (dpi). Results: A statistically significant increase was demonstrated in the tlr4 mRNA level isolated from the infected mice jejunum at 4, 8, and 16 dpi over the uninfected control. Moreover, at 4, 8, and 16 dpi in the jejunum of infected mice, a strong positive reaction for the presence of TLR4 protein compared with that of uninfected mice was observed. Conclusion: Infection with T. spiralis changes the expression of the tlr4 gene in the small intestine of the mouse host. |
| Databáze: | OpenAIRE |
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