Protein Profiles of Bronchoalveolar Lavage Fluid from Patients with Pulmonary Sarcoidosis

Autor: Annett Bleul, Roland M. du Bois, Frantisek Mrazek, Christian Melle, Vitezslav Kolek, Eva Kriegova, Beata Hutyrova, Ferdinand von Eggeling, Martin Petrek
Rok vydání: 2006
Předmět:
Adult
Male
Pulmonary and Respiratory Medicine
Resuscitation
Systemic disease
Pathology
medicine.medical_specialty
education
Protein Array Analysis
Down-Regulation
Critical Care and Intensive Care Medicine
Peptide Mapping
Sensitivity and Specificity
Severity of Illness Index
Löfgren syndrome
Statistics
Nonparametric
Sarcoidosis
Pulmonary
Reference Values
Intensive care
Macrophages
Alveolar
Humans
Medicine
Stage (cooking)
health care economics and organizations
Aged
Probability
medicine.diagnostic_test
business.industry
Albumin
Proteins
Middle Aged
medicine.disease
Respiratory Function Tests
Up-Regulation
Bronchoalveolar lavage
Case-Control Studies
Spectrometry
Mass
Matrix-Assisted Laser Desorption-Ionization
Disease Progression
Female
Sarcoidosis
business
Bronchoalveolar Lavage Fluid
Biomarkers
Zdroj: American Journal of Respiratory and Critical Care Medicine. 173:1145-1154
ISSN: 1535-4970
1073-449X
Popis: Pulmonary sarcoidosis is a multisystem granulomatous disease with various clinical phenotypes. So far, there has been little information on protein patterns (PPs) of bronchoalveolar lavage fluid (BALF) from patients with sarcoidosis and no data are available on PPs in clinical disease subtypes.To investigate the PP of BALF from patients with pulmonary sarcoidosis, to evaluate whether PPs reflect disease course as assessed by chest X-ray (CXR), and to compare PPs between patients with/without Löfgren's syndrome.Surface-enhanced laser desorption/ionization-time-of-flight mass spectroscopy was applied to investigate PPs in unconcentrated BALF from 65 patients (CXR stage I, n = 32; CXR stage II, n = 22, CXR stage III, n = 11) and 23 healthy control subjects. The Mann-Whitney U test was used to detect differentially expressed protein peaks. After reversed-phase fractionation, peptide fingerprint mapping and immunodepletion were used to identify deregulated (up-regulated or down-regulated) proteins.Forty differentially expressed protein entities (2.75-185.62 kD) were detected in patients with pulmonary sarcoidosis versus control subjects (p0.05). Whereas 13 peaks (33%) were present across all CXR stages, 27 (67%) were specific for particular CXR stages. Comparison of PPs between CXR stage I patients with or without Löfgren's syndrome revealed 25 differentially expressed peaks. The total number of deregulated peaks and also of those associated with sarcoidosis as a whole were markedly lower in patients with Löfgren's syndrome in comparison with other sarcoid phenotypes. Human serum albumin, alpha1-antitrypsin, and protocadherin-2 precursor were identified from sarcoidosis-associated PP.Surface-enhanced laser desorption/ionization-time-of-flight mass spectroscopy enables determination of protein patterns in sarcoid BALF and allows detection of protein patterns linked to a particular disease course.
Databáze: OpenAIRE
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