Evaluation of the expanded measurement uncertainty in the determination of the net flux of nutrients and carbon atoms through the liver in ruminants
| Autor: | Denys Durand, Gonzalo Cantalapiedra-Hijar, Max Feinberg, Agnès Isserty-Thomas, Isabelle Ortigues-Marty, J. M. Rodríguez-López |
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| Přispěvatelé: | Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Méthodologies d'Analyse de Risque Alimentaire (MET@RISK), Institut National de la Recherche Agronomique (INRA), REDNEX EU FP7 Project, Adisseo France SAS, VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
General Chemical Engineering Analytical chemistry chemistry.chemical_element Hematocrit Matrix (chemical analysis) 03 medical and health sciences Flux (metallurgy) Nutrient hepatic flux [CHIM.ANAL]Chemical Sciences/Analytical chemistry measurement uncertainty medicine blood flow Safety Risk Reliability and Quality Instrumentation validation Measurement method 030109 nutrition & dietetics medicine.diagnostic_test Chemistry 0402 animal and dairy science 04 agricultural and veterinary sciences General Chemistry Blood flow 040201 dairy & animal science carbon flux Environmental chemistry Measurement uncertainty Carbon |
| Zdroj: | Accreditation and Quality Assurance Accreditation and Quality Assurance, Springer Verlag, 2016, 21 (2), pp.131-142. ⟨10.1007/s00769-015-1187-3⟩ |
| ISSN: | 0949-1775 1432-0517 |
| DOI: | 10.1007/s00769-015-1187-3⟩ |
| Popis: | International audience; In in vivo metabolic studies, quantifying the flux of a nutrient and/or the carbon atoms through the liver implies the analytical determination of nutrient concentrations before and after crossing the liver and of blood or plasma flux. There is a measurement uncertainty (MU) associated with each input quantity, which may compromise accurate physiological interpretations of the results. The objective of this work was to calculate the expanded MU of net fluxes of nutrients and carbon atoms through the liver of ruminants and to identify the main components that affect MUs. To this end, all quantities (i.e., nutrient concentrations, para-aminohippuric acid concentrations, hematocrit, time of infusion, and others) that influenced the final results were identified and quantified. Their associated MUs were calculated with Type A or Type B approaches. An expanded MU model was developed by combining these two approaches. In general, the relative expanded MU of the net flux of nutrients calculated in plasma was lower than that in blood. For both plasma and blood fluxes, the main components that increased MUs were identified. The net hepatic flux of carbon atoms, for example, was estimated to be (63 +/- A 229) mmol min(-1). This high expanded MU was mainly a result of acetate concentration measurements in hepatic and portal veins. Priority strategies to reduce MUs, such as selecting an adequate matrix or using more accurate measurement methods, were identified. |
| Databáze: | OpenAIRE |
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