How to overcome the ATRA resistance with the 9aaTAD activation domains in retinoic acid receptors
| Autor: | Marek Havelka, Martin Piskacek, Andrea Knight |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0303 health sciences
Peroxisome proliferator-activated receptor gamma Mutant Retinoic acid Calcitriol receptor Cell biology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Hepatocyte nuclear factor 4 chemistry Nuclear receptor Transcription (biology) 030220 oncology & carcinogenesis Receptor 030304 developmental biology |
| DOI: | 10.1101/298646 |
| Popis: | In higher metazoa, the nuclear hormone receptors activate transcription trough their specific adaptors, nuclear hormone receptor cofactors NCoA, which are surprisingly absent in lower metazoa. In this study, we demonstrated that the 9aaTAD from NHR-49 receptor activates transcription as a small peptide. We showed, that the 9aaTAD domains are conserved in the human nuclear hormone receptors including HNF4, RARa, VDR and PPARg. The small 9aaTAD peptides derived from these nuclear hormone receptors also effectively activated transcription and that in absence of the NCoA adaptors. We identified adjacent inhibitory domains in the human HNF4 and RARa, which hindered their activation function.In acute promyelocytic leukaemia (PML-RARa), the receptor mutations often caused all-trans retinoic acid (ATRA) resistance. The fact that almost the entire receptor is needed for ATRA mediated receptor activation, this activation pathway is highly susceptible for loss of function when mutated. Nevertheless in the most of the reported mutants, the activation domains 9aaTAD are still intact. The release of activation 9aaTAD from its dormancy by a new drug might be the sound strategy in combat the ATRA resistance in PML leukaemia.Graphical Abstract |
| Databáze: | OpenAIRE |
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