The structure of the capsular polysaccharide of Shewanella oneidensis strain MR-4
| Autor: | Ludmila Nossova, Anton Korenevsky, Evgeny Vinogradov, Terry J. Beveridge |
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| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Shewanella
STRAIN Magnetic Resonance Spectroscopy Stereochemistry Molecular Sequence Data Polysaccharide Biochemistry Analytical Chemistry Residue (chemistry) Monosaccharide structure Shewanella oneidensis chemistry.chemical_classification Chromatography biology Strain (chemistry) Polysaccharides Bacterial Organic Chemistry General Medicine Carbon-13 NMR biology.organism_classification capsular polysaccharide POLYSACCHARIDE Carbohydrate Sequence chemistry Pyranose |
| Popis: | Capsular polysaccharides were extracted from Shewanella oneidensis strain MR-4, grown on two different culture media. The polysaccharides were analyzed using 1 H and 13 C NMR spectroscopy, and the following structure of the repeating unit was established: where the residue of 4-amino-4,6-dideoxy- D -glucose (Qui4N) was substituted with different N -acyl groups depending on the growth media. All monosaccharides are present in the pyranose form. In the PS from cells grown on enriched medium (trypticase soy broth, TSB) aerobically it was N-acylated with 3-hydroxy-3-methylbutyrate (60%) or with 3-hydroxybutyrate (40%), whereas in the PS from cells grown on minimal medium (CDM) aerobically it was acylated mostly with 3-hydroxybutyrate (>90%). |
| Databáze: | OpenAIRE |
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