Assessment of Enzyme Inhibitory and Antioxidant Activities of Lignans from Taxus baccata L
| Autor: | Ilkay Erdogan Orhan, Bilge Sener, M. Iqbal Choudhary, Nurgün Küçükboyaci, S Ahmad Nawaz |
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| Rok vydání: | 2010 |
| Předmět: |
Antioxidant
Turkey DPPH medicine.medical_treatment Antioxidants General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Chlorogenic acid Alzheimer Disease medicine Humans Lipoxygenase Inhibitors Enzyme Inhibitors Butyrylcholinesterase Lariciresinol Plants Medicinal biology Free Radical Scavengers biology.organism_classification Enzyme assay chemistry Phytochemical Taxus Biochemistry biology.protein Cholinesterase Inhibitors |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1865-7125 0939-5075 |
| Popis: | Phytochemical investigations of Taxus baccata L. by successive chromatographic methods resulted in the isolation of the lignans lariciresinol (1), taxiresinol (2), 3´-demethylisolariciresinol- 9´-hydroxyisopropylether (3), isolariciresinol (4), and 3-demethylisolariciresinol (5) as well as taxoids. Compounds 1 - 5 were evaluated for their acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and lipoxygenase (LOX) inhibitory activities, which play a role in the pathogenesis of Alzheimer’s disease (AD), by in vitro spectrophotometric methods, while they were also screened for their antioxidant capacity in 2,2-diphenyl-1- picrylhydrazyl (DPPH) radical scavenging, ferrous ion-chelating effect, and ferric-reducing antioxidant power (FRAP) at 125, 250, 500, and 1000 μg ml-1. All compounds exhibited a moderate inhibition against both BChE and LOX, whereas they were inactive towards AChE. The compounds displayed a great scavenging activity against DPPH especially at 500 and 1000 μg ml-1. Besides, they were found to exert noteworthy reducing antioxidant power on ferric ions. In particular, the FRAP of compounds 2 (3.552 ± 0.02), 4 (3.021 ± 0.71), and 5 (3.533 ± 0.01) were as high as that of the reference chlorogenic acid (3.618 ± 0.01) at 1000 μg ml-1. None of the compounds exhibited chelating ability against ferrous ions. |
| Databáze: | OpenAIRE |
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