Expression of Potential Regulatory Genes in Abdominal Adipose Tissue of Broiler Chickens during Early Development
| Autor: | Sri Harsha K. V. Subramanya, Gary Kelley, James Tyus, Ashley Payne, Dafeng Hui, Shannon Byers, Fur-Chi Chen, Samuel N. Nahashon, Xiaofei Wang, Michael T. Ivy, Joseph Donkor, Carl E. Darris, Ann Bohannon-Stewart, Boniface M. Kimathi |
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| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | Genetics Research International Genetics Research International, Vol 2014 (2014) |
| ISSN: | 2090-3162 2090-3154 |
| DOI: | 10.1155/2014/318304 |
| Popis: | The identities of genes that underlie population variation in adipose tissue development in farm animals are poorly understood. Previous studies in our laboratory have suggested that increased fat tissue involves the expression modulation of an array of genes in broiler chickens. Of special interest are eight genes,FGFR3, EPHB2, IGFBP2, GREM1, TNC, COL3A1, ACBD7, andSCD. To understand their expression regulation and response to dietary manipulation, we investigated their mRNA levels after dietary manipulation during early development. Chickens were fed either a recommended standard or a high caloric diet from hatch to eight weeks of age (WOA). The high caloric diet markedly affected bodyweight of the broiler birds. mRNA levels of the eight genes in the abdominal adipose tissue were assayed at 2, 4, 6, and 8 WOA using RT-qPCR. Results indicate that (1)FGFR3mRNA level was affected significantly by diet, age, and diet:age interaction; (2)COL3AmRNA level was repressed by high caloric diet; (3) mRNA levels ofEPHB2, ACBD7, andSCDwere affected by age; (4) mRNA level ofTNCwas modulated by age:diet interaction; (5) changes inGREM1andIGFBP2mRNA levels were not statistically different. |
| Databáze: | OpenAIRE |
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