Long-Term Effects of the Abdominal Aortic Aneurysm Model in Rabbits Prepared by Pancreatic Elastase Combined With Angiotensin II
| Autor: | Qing Chen, Mao-Xiao Nie, Quan-Ming Zhao |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
medicine.medical_specialty Time Factors Urology H&E stain Vascular Remodeling 030204 cardiovascular system & hematology 030218 nuclear medicine & medical imaging 03 medical and health sciences 0302 clinical medicine Aneurysm Animals Medicine Aorta Abdominal Ultrasonography Doppler Color Pancreatic elastase Pancreatic Elastase business.industry Angiotensin II Elastase Hemodynamics General Medicine medicine.disease Abdominal aortic aneurysm Disease Models Animal Matrix Metalloproteinase 9 Disease Progression cardiovascular system Matrix Metalloproteinase 2 Immunohistochemistry Surgery Rabbits Cardiology and Cardiovascular Medicine business Perfusion Aortic Aneurysm Abdominal Dilatation Pathologic |
| Zdroj: | Vascular and Endovascular Surgery. 53:35-41 |
| ISSN: | 1938-9116 1538-5744 |
| Popis: | Objective: This study aimed to observe the effect of pancreatic elastase combined with angiotensin II on a stable rabbit abdominal aortic aneurysm model. Methods: A total of 20 male New Zealand rabbits were randomly divided into groups A and B, with 10 rabbits per group. The rabbits in group A were given an intraperitoneal perfusion of pancreatic elastase, and the rabbits in group B were given continuous pumping of angiotensin II in addition to the operation of group A. Before the operation and at 2, 4, and 16 weeks postoperation, vascular color Doppler ultrasonography was performed, and blood samples were collected to measure the serum matrix metalloproteinase 9 (MMP9) and MMP2 levels. At 16 weeks postoperation, all rabbits in both groups were killed, and hematoxylin and eosin, Elastic-van-Gieson, Masson’s, and immunohistochemical staining were performed for the vessel specimens. Results: At 2 weeks postoperation, the aneurysm formation rates of the 2 groups were both 100%, and the average expansion rates of the aneurysm diameters were 85% and 93%, respectively; these differences were not significant ( P = .150 and P = .280, respectively). At 4 weeks postoperation, the aneurysm formation rates of the 2 groups were 71.4% and 100%, and the average expansion rates of the aneurysm diameter were 68% and 99%, respectively; the differences between the groups were significant ( P = .031 and P = .022, respectively). At 16 weeks postoperation, the aneurysm formation rates of the 2 groups were 14.3% and 100%, and the average expansion rates of the aneurysm diameter were 12% and 108%, respectively; the differences between the groups were significant ( P = .026 and P = .014, respectively). Conclusion: Compared to the abdominal aortic aneurysm modeling method in rabbits based on pancreatic elastase alone, the abdominal aortic aneurysm modeling method in rabbits using pancreatic elastase combined with angiotensin II maintained the morphology of the abdominal aortic aneurysm for a longer time, showing an important application value for the long-term observation of changes in abdominal aortic aneurysms. |
| Databáze: | OpenAIRE |
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