Immune response and Raman scattering assessment in rats skin after contact with Fusarium oxysporum metabolites
Autor: | Francielle Sato, Gustavo Henrique Doná Rodrigues de Almeida, Terezinha Ines Estivalet Svidzinski, Mauro Luciano Baesso, Gutierrez Rodrigues de Moraes, Kátia Cristina Sibin Melo, Marcelo Henrique Correia, Mariana Sversut Gibin, Antonio M. Bento, Ciomar Aparecida Bersani Amado, Luzmarina Hernandes |
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Rok vydání: | 2020 |
Předmět: |
Male
Fusarium Injections Intradermal Inflammation 02 engineering and technology Spectrum Analysis Raman 010402 general chemistry 01 natural sciences Analytical Chemistry Transforming Growth Factor beta1 Subcutaneous Tissue Immune system Dermis Fusarium oxysporum medicine Animals Rats Wistar Instrumentation Spectroscopy Skin chemistry.chemical_classification Principal Component Analysis biology Interleukin-6 Tumor Necrosis Factor-alpha 021001 nanoscience & nanotechnology biology.organism_classification Molecular biology Atomic and Molecular Physics and Optics 0104 chemical sciences medicine.anatomical_structure Enzyme chemistry Metabolome Immunohistochemistry medicine.symptom 0210 nano-technology Immunostaining |
Zdroj: | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 234:118246 |
ISSN: | 1386-1425 |
Popis: | The secondary metabolites produced by Fusarium can cause disease and death when consumed and produce biological responses even in the absence of the microorganism. The IL-6, TNF-α and TGF-β1 cytokines immune reactivity was associated with histopathological and physico-chemical changes in skin of immune competent rats after administration of Fusarium oxysporum crude extract. Rats were intradermally injected with 50 μl of 0.5 mg/ml crude extract and were euthanized at 3, 6, 12 and 24 h after injection. The inflammatory response was quantified by enzyme myeloperoxidase activity and by immunohistochemical method to detect the IL-6, TNF-α and TGF-β1. Physico-chemical analysis was performed using FT-Raman Spectroscopy. The inflammatory response was most intense at 6 and 12 h after crude extract administration and the most significant histopathological changes were observed in the dermis. Myeloperoxidase activity was intense from 3 to 24 h after injection. The immunostaining of pro-inflammatory cytokines IL-6 and TNF-α peaked at 6 h. Immunostaining for TGF-β1 was highest at 12 and 24 h. FT-Raman spectral analysis showed both, the most intense Fusarium interaction with the skin at 6 h, as revealed by the changes in the stretching of –CH bands (3100–2800 cm−1) in the dermis, and skin recovery trending after 12 h after crude extract injection. The results showed that secondary metabolites stimulated histopathologic changes and inflammatory responses even in the absence of the fungus, increasing myeloperoxidase activity and pro-inflammatory cytokine expression besides promoting physico-chemical changes. |
Databáze: | OpenAIRE |
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