Intravenous Grafts Of Amniotic Fluid-Derived Stem Cells Induce Endogenous Cell Proliferation and Attenuate Behavioral Deficits in Ischemic Stroke Rats

Autor: Liborio Stuppia, Yuji Kaneko, Paula C. Bickford, Isao Date, Cesar V. Borlongan, Loren E. Glover, Alejandra Jacotte Simancas, Sandra Acosta, Marianna A. Solomita, Naoki Tajiri, Takao Yasuhara, Ivana Antonucci
Rok vydání: 2012
Předmět:
Male
Pathology
Time Factors
Amniotic fluid
medicine.medical_treatment
H&E stain
Cardiovascular
Rats
Sprague-Dawley
Neural Stem Cells
Ischemia
Pregnancy
Lateral Ventricles
Stroke
Multidisciplinary
Behavior
Animal
Stem Cells
Infarction
Middle Cerebral Artery
Amniotic stem cells
Animal Models
Stem-cell therapy
Immunohistochemistry
Motor Skills Disorders
medicine.anatomical_structure
Neurology
Medicine
Female
Microtubule-Associated Proteins
Research Article
medicine.medical_specialty
Science
Cerebrovascular Diseases
Subventricular zone
Model Organisms
Developmental Neuroscience
medicine
Animals
cardiovascular diseases
Biology
Cell Proliferation
Ischemic Stroke
business.industry
Dentate gyrus
Social Behavior Disorders
Recovery of Function
Amniotic Fluid
medicine.disease
Rats
Ki-67 Antigen
Dentate Gyrus
Rat
business
Stem Cell Transplantation
Developmental Biology
Neuroscience
Zdroj: PLoS ONE
PLoS ONE, Vol 7, Iss 8, p e43779 (2012)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0043779
Popis: We recently reported isolation of viable rat amniotic fluid-derived stem (AFS) cells [1]. Here, we tested the therapeutic benefits of AFS cells in a rodent model of ischemic stroke. Adult male Sprague-Dawley rats received a 60-minute middle cerebral artery occlusion (MCAo). Thirty-five days later, animals exhibiting significant motor deficits received intravenous transplants of rat AFS cells or vehicle. At days 60-63 post-MCAo, significant recovery of motor and cognitive function was seen in stroke animals transplanted with AFS cells compared to vehicle-infused stroke animals. Infarct volume, as revealed by hematoxylin and eosin (H&E) staining, was significantly reduced, coupled with significant increments in the cell proliferation marker, Ki67, and the neuronal marker, MAP2, in the dentate gyrus (DG) [2] and the subventricular zone (SVZ) of AFS cell-transplanted stroke animals compared to vehicle-infused stroke animals. A significantly higher number of double-labeled Ki67/MAP2-positive cells and a similar trend towards increased Ki67/MAP2 double-labeling were observed in the DG and SVZ of AFS cell-transplanted stroke animals, respectively, compared to vehicle-infused stroke animals. This study reports the therapeutic potential of AFS cell transplantation in stroke animals, possibly via enhancement of endogenous repair mechanisms.
Databáze: OpenAIRE
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