Poly-γ-Glutamic Acid: Biodegradable Polymer for Potential Protection of Beneficial Viruses
| Autor: | Ibrahim R Khalil, Iza Radecka, Martin Khechara, Jessica L Mason, Victor U. Irorere, Alan T. H. Burns, Marek Kowalczuk |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
γ-PGA viruses 030106 microbiology lcsh:Technology Article Microbiology Bacteriophage 03 medical and health sciences bacteriophage General Materials Science Irradiation lcsh:Microscopy lcsh:QC120-168.85 chemistry.chemical_classification lcsh:QH201-278.5 biology lcsh:T biodegradable polymer Polymer Glutamic acid biology.organism_classification Biodegradable polymer 030104 developmental biology chemistry lcsh:TA1-2040 lcsh:Descriptive and experimental mechanics lcsh:Electrical engineering. Electronics. Nuclear engineering lcsh:Engineering (General). Civil engineering (General) Desiccation lcsh:TK1-9971 |
| Zdroj: | Materials, Vol 9, Iss 1, p 28 (2016) Materials Materials; Volume 9; Issue 1; Pages: 28 |
| ISSN: | 1996-1944 |
| DOI: | 10.3390/ma9010028 |
| Popis: | Poly-γ-glutamic acid (γ-PGA) is a naturally occurring polymer, which due to its biodegradable, non-toxic and non-immunogenic properties has been used successfully in the food, medical and wastewater industries. A major hurdle in bacteriophage application is the inability of phage to persist for extended periods in the environment due to their susceptibility to environmental factors such as temperature, sunlight, desiccation and irradiation. Thus, the aim of this study was to protect useful phage from the harmful effect of these environmental factors using the γ-PGA biodegradable polymer. In addition, the association between γ-PGA and phage was investigated. Formulated phage (with 1% γ-PGA) and non-formulated phage were exposed to 50 °C. A clear difference was noticed as viability of non-formulated phage was reduced to 21% at log10 1.3 PFU/mL, while phage formulated with γ-PGA was 84% at log10 5.2 PFU/mL after 24 h of exposure. In addition, formulated phage remained viable at log10 2.5 PFU/mL even after 24 h of exposure at pH 3 solution. In contrast, non-formulated phages were totally inactivated after the same time of exposure. In addition, non-formulated phages when exposed to UV irradiation died within 10 min. In contrast also phages formulated with 1% γ-PGA had a viability of log10 4.1 PFU/mL at the same exposure time. Microscopy showed a clear interaction between γ-PGA and phages. In conclusion, the results suggest that γ-PGA has an unique protective effect on phage particles. |
| Databáze: | OpenAIRE |
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