The Traditional Chinese Medicine DangguiBuxue Tang Sensitizes Colorectal Cancer Cells to Chemoradiotherapy
| Autor: | Ming Cheng Lee, Chih Wen Chi, Tzung Yan Lee, Yu-Jen Chen, Tung Hu Tsai, Shun Ting Chen, Hen-Hong Chang, Ming Ling Hsu, Hui Ru Shieh, Chin Ping Lin, Yin Cheng Lin |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Oncology Programmed cell death medicine.medical_specialty DangguiBuxue Tang Colorectal cancer medicine.medical_treatment Pharmaceutical Science colorectal cancer Vacuole chemotherapy Radiation Tolerance Article Analytical Chemistry lcsh:QD241-441 03 medical and health sciences Inhibitory Concentration 50 0302 clinical medicine lcsh:Organic chemistry Internal medicine Drug Discovery medicine Humans Physical and Theoretical Chemistry radiotherapy Chemotherapy business.industry Organic Chemistry Therapeutic effect Drug Synergism Chemoradiotherapy medicine.disease Antineoplastic Agents Phytogenic Radiation therapy 030104 developmental biology Vacuolization Chemistry (miscellaneous) Drug Resistance Neoplasm 030220 oncology & carcinogenesis Cancer research Molecular Medicine Fluorouracil Drug Screening Assays Antitumor business HT29 Cells Drugs Chinese Herbal |
| Zdroj: | Molecules Molecules; Volume 21; Issue 12; Pages: 1677 Molecules, Vol 21, Iss 12, p 1677 (2016) |
| ISSN: | 1420-3049 |
| Popis: | Chemotherapy is an important treatment modality for colon cancer, and concurrent chemoradiation therapy (CCRT) is the preferred treatment route for patients with stage II and III rectal cancer. We examined whether DangguiBuxue Tang (DBT), a traditional Chinese herbal extract, sensitizes colorectal cancer cells to anticancer treatments. The polysaccharide-depleted fraction of DBT (DBT-PD) contains greater amounts of astragaloside IV (312.626 µg/g) and ferulic acid (1.404 µg/g) than does the original formula. Treatment of the murine colon carcinoma cell line (CT26) with DBT-PD inhibits growth, whereas treatment with comparable amounts of purified astragaloside IV and ferulic acid showed no significant effect. Concurrent treatment with DBT-PD increases the growth inhibitory effect of 5-fluorouracil up to 4.39-fold. DBT-PD enhances the effect of radiation therapy (RT) with a sensitizer enhancement ratio (SER) of up to 1.3. It also increases the therapeutic effect of CCRT on CT26 cells. Cells treated with DBP-PD showed ultrastructural changes characteristic of autophagy, including multiple cytoplasmic vacuoles with double-layered membranes, vacuoles containing remnants of degraded organelles, marked swelling and vacuolization of mitochondria, and autolysosome-like vacuoles. We conclude that DBT-PD induces autophagy-associated cell death in CT26 cells, and may have potential as a chemotherapy or radiotherapy sensitizer in colorectal cancer treatment. |
| Databáze: | OpenAIRE |
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