Three-dimensional Quantification of Dendritic Spines from Pyramidal Neurons Derived from Human Induced Pluripotent Stem Cells
| Autor: | Isabelle Cloëz-Tayarani, Thomas Bourgeron, Alexandra Benchoua, Laura Gouder, Hany Goubran-Botros, Jean-Yves Tinevez |
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| Přispěvatelé: | Université Paris Diderot - Paris 7 (UPD7), Gènes, Synapses et Cognition (CNRS - UMR3571 ), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Génétique humaine et fonctions cognitives - Human Genetics and Cognitive Functions (GHFC (UMR_3571 / U-Pasteur_1)), Institut Pasteur [Paris] (IP)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Imagerie Dynamique (Plate-Forme) (PFID), Institut Pasteur [Paris] (IP), Institut des cellules souches pour le traitement et l'étude des maladies monogéniques (I-STEM), Université d'Évry-Val-d'Essonne (UEVE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Généthon, This work was funded by the Institut Pasteur, the Bettencourt-Schueller foundation, Centre National de la Recherche Scientifique, University Paris Diderot, Agence Nationale de la Recherche (ANR-13-SAMA-0006, SynDivAutism), the Conny-Maeva Charitable Foundation, the Cognacq Jay Foundation, the Orange Foundation, and the Fondamental Foundation. L.G. is supported by an undergraduate fellowship from the Health Ministry., We acknowledge the help of BitPlane in particular Georgia Golfis, in the early stage of this work., ANR-13-SAMA-0006,SynDivAutism,Diversité Synaptique dans l'autisme(2013), Gènes, Synapses et Cognition, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris]-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris], Université d'Évry-Val-d'Essonne (UEVE)-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Pluripotent Stem Cells
MESH: Neural Stem Cells Dendritic spine General Chemical Engineering Dendritic Spines [SDV]Life Sciences [q-bio] Green Fluorescent Proteins Induced Pluripotent Stem Cells Glutamic Acid Biology MESH: Induced Pluripotent Stem Cells MESH: Dendrites Receptors N-Methyl-D-Aspartate General Biochemistry Genetics and Molecular Biology MESH: Synapses MESH: Dendritic Spines Glutamatergic [SCCO]Cognitive science MESH: Green Fluorescent Proteins Neural Stem Cells Postsynaptic potential medicine Premovement neuronal activity Humans MESH: Microscopy Confocal Induced pluripotent stem cell MESH: Receptors N-Methyl-D-Aspartate [SDV.GEN]Life Sciences [q-bio]/Genetics Microscopy Confocal MESH: Humans General Immunology and Microbiology General Neuroscience Pyramidal Cells Dendrites MESH: Glutamic Acid MESH: Pyramidal Cells Neural stem cell medicine.anatomical_structure [SDV.MHEP.PSM]Life Sciences [q-bio]/Human health and pathology/Psychiatrics and mental health Synapses MESH: Pluripotent Stem Cells Neuron Stem cell Neuroscience Developmental Biology |
| Zdroj: | Journal of visualized experiments : JoVE Journal of visualized experiments : JoVE, 2015, 104, pp.e53197. ⟨10.3791/53197⟩ Journal of visualized experiments : JoVE, JoVE, 2015, pp.e53197. ⟨10.3791/53197⟩ |
| ISSN: | 1940-087X |
| DOI: | 10.3791/53197⟩ |
| Popis: | International audience; Dendritic spines are small protrusions that correspond to the post-synaptic compartments of excitatory synapses in the central nervous system. They are distributed along the dendrites. Their morphology is largely dependent on neuronal activity, and they are dynamic. Dendritic spines express glutamatergic receptors (AMPA and NMDA receptors) on their surface and at the levels of postsynaptic densities. Each spine allows the neuron to control its state and local activity independently. Spine morphologies have been extensively studied in glutamatergic pyramidal cells of the brain cortex, using both in vivo approaches and neuronal cultures obtained from rodent tissues. Neuropathological conditions can be associated to altered spine induction and maturation, as shown in rodent cultured neurons and one-dimensional quantitative analysis (1). The present study describes a protocol for the 3D quantitative analysis of spine morphologies using human cortical neurons derived from neural stem cells (late cortical progenitors). These cells were initially obtained from induced pluripotent stem cells. This protocol allows the analysis of spine morphologies at different culture periods, and with possible comparison between induced pluripotent stem cells obtained from control individuals with those obtained from patients with psychiatric diseases. |
| Databáze: | OpenAIRE |
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