Ouabain sensitivity of a chimeric alpha subunit (Torpedo/rat) of the (Na,K)ATPase expressed in Xenopus oocyte
| Autor: | Kazuo Takeda, Michihiro Ohtsubo, Toshiko Ohta, Shunsuke Noguchi, Masaru Kawamura |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
ATPase
Xenopus Molecular Sequence Data Biophysics Torpedo Biochemistry Ouabain law.invention law Salientia polycyclic compounds medicine Animals Amino Acid Sequence Na+/K+-ATPase Molecular Biology Peptide sequence biology Base Sequence Chimera Cell Biology biology.organism_classification Molecular biology Transmembrane protein Rats biology.protein Oocytes Sodium-Potassium-Exchanging ATPase medicine.drug |
| Zdroj: | Biochemical and biophysical research communications. 155(3) |
| ISSN: | 0006-291X |
| Popis: | A cDNA for a chimeric alpha subunit of the (Na,K)ATPase was constructed and expressed in Xenopus oocytes in order to elucidate structural features involved in ouabain sensitivity. A chimeric alpha subunit, in which the N-terminal 165 amino acid sequence of ouabain-resistant rat alpha subunit, including the first two transmembrane segments (M1 and M2), was replaced by a sequence from the corresponding region of ouabain-sensitive Torpedo alpha subunit, was ouabain-sensitive, suggesting that the M1-M2 junction is a site responsible for ouabain sensitivity of the (Na,K)ATPase. |
| Databáze: | OpenAIRE |
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