The Effects of Human Bone Marrow-Derived Mesenchymal Stem Cell Conditioned Media Produced with Fetal Bovine Serum or Human Platelet Lysate on Skin Rejuvenation Characteristics
| Autor: | JeeHoon Nam, Eunkyung Chung, Kim Sina, Lee Chanju, Sun U. Song, Byeol Choi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Fetal bovine serum
Human mesenchymal stem cell Extracellular matrix Dermal fibroblast 03 medical and health sciences 0302 clinical medicine medicine Human platelet lysate Wrinkle 030304 developmental biology Skin rejuvenation 0303 health sciences biology integumentary system Chemistry Regeneration (biology) Mesenchymal stem cell Cell Biology Cell biology Secretory protein biology.protein Original Article medicine.symptom Elastin 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | International Journal of Stem Cells |
| ISSN: | 2005-5447 2005-3606 |
| Popis: | Background and objectives Human mesenchymal stem cell-conditioned medium (MSC-CM) is produced using mesenchymal stem cell culture technology and has various benefits for the skin, including wrinkle removal, skin regeneration, and increased antioxidant activity. Its popularity is thus increasing in the field of functional cosmetics. Methods and results In this study, we analyzed the effects of fetal bovine serum-supplemented MSC-CM (FBSMSC-CM) and human platelet lysate-supplemented MSC-CM (hPL-MSC-CM) on skin rejuvenation characteristics. We found that the concentrations of important growth factors (VEGF, TGF-β1, and HGF) and secretory proteins for skin regeneration were significantly higher in hPL-MSC-CM than in FBS-MSC-CM. Furthermore, the capacity for inducing proliferation of human dermal fibroblast (HDF) and keratinocytes, the migration ability of HDF, extracellular matrix (ECM) production such as collagen and elastin was higher in hPL-MSC-CM than that in FBSMSC-CM. Conclusions These results support the usefulness and high economic value of hPL-MSC-CM as an alternative source of FBS-MSC-CM in the cosmetic industry for skin rejuvenation. |
| Databáze: | OpenAIRE |
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