Functional Changes in Aging Polymorphonuclear Leukocytes
| Autor: | James C. Hogg, Mitsushi Okazawa, Kayoko Tanji-Matsuba, Yuji Saito, Shizu Hayashi, Stephan F. van Eeden, Maria E. Klut |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
medicine.medical_specialty
Neutrophils Cell Apoptosis Stimulation In Vitro Techniques Granulocyte Biology Filamentous actin Physiology (medical) Internal medicine Polymorphonuclear Neutrophils medicine Animals L-Selectin Cellular Senescence Cell adhesion molecule hemic and immune systems Chemotaxis Hydrogen Peroxide Actins N-Formylmethionine Leucyl-Phenylalanine Endocrinology medicine.anatomical_structure CD18 Antigens Immunology Female Rabbits Cardiology and Cardiovascular Medicine |
| Zdroj: | Circulation. 97:91-98 |
| ISSN: | 1524-4539 0009-7322 |
| DOI: | 10.1161/01.cir.97.1.91 |
| Popis: | Background —Previous studies from our laboratory have shown that the expression of L-selectin on polymorphonuclear neutrophils (PMN) decreases as the cell ages in the circulation and that these older PMN have more fragmented DNA and show morphological features of apoptosis. Methods and Results —The present study was designed to compare the functional capabilities of PMN expressing low levels of L-selectin (L-selectin low ) and the total population of PMN they were isolated from (L-selectin mixed ). The results show no difference of the baseline filamentous actin (F-actin) content between PMN expressing low and high levels of L-selectin. However, the ability of L-selectin low PMN to assemble F-actin was impaired after stimulation by n -formyl- l -methionyl- l -leucyl- l -phenylalanine (fMLP) (1 nmol/L fMLP: P P low PMN to change shape when stimulated (10 nmol/L fMLP) was also decreased ( P low and L-selectin mixed leukocytes, but the L-selectin low cells showed a decreased ability to stiffen after fMLP stimulation ( P low cells demonstrated a decreased ability to migrate toward a chemoattractant (1, 3, and 10 nmol/L fMLP) ( P P P Conclusions —We conclude that PMN undergo substantial functional changes as they age in the circulation. |
| Databáze: | OpenAIRE |
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