3D Printed Structures Filled with Carbon Fibers and Functionalized with Mesenchymal Stem Cell Conditioned Media as In Vitro Cell Niches for Promoting Chondrogenesis
| Autor: | Andrés Díaz Lantada, Josefa P. García-Ruiz |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Scaffold engineering Biología Cell 02 engineering and technology scaffolds for tissue repair lcsh:Technology Article Ingeniería Industrial carbon fiber computer-aided design & engineering 03 medical and health sciences Tissue engineering computer-aided design & medicine General Materials Science lcsh:Microscopy Cell adhesion lcsh:QC120-168.85 rapid prototyping Materiales lcsh:QH201-278.5 lcsh:T Chemistry Regeneration (biology) Mesenchymal stem cell cell culture niches 021001 nanoscience & nanotechnology Chondrogenesis Cell biology 030104 developmental biology medicine.anatomical_structure lcsh:TA1-2040 Cell culture tissue engineering stem cell niches stem cell conditioned medium additive manufacture lcsh:Descriptive and experimental mechanics lcsh:Electrical engineering. Electronics. Nuclear engineering lcsh:Engineering (General). Civil engineering (General) 0210 nano-technology lcsh:TK1-9971 Mecánica |
| Zdroj: | Materials, ISSN 1996-1944, 2018-01, Vol. 11, No. 1 Archivo Digital UPM Universidad Politécnica de Madrid Materials Materials; Volume 11; Issue 1; Pages: 23 Materials, Vol 11, Iss 1, p 23 (2017) |
| Popis: | In this study, we present a novel approach towards the straightforward, rapid, and low-cost development of biomimetic composite scaffolds for tissue engineering strategies. The system is based on the additive manufacture of a computer-designed lattice structure or framework, into which carbon fibers are subsequently knitted or incorporated. The 3D-printed lattice structure acts as support and the knitted carbon fibers perform as driving elements for promoting cell colonization of the three-dimensional construct. A human mesenchymal stem cell (h-MSC) conditioned medium (CM) is also used for improving the scaffold’s response and promoting cell adhesion, proliferation, and viability. Cell culture results—in which scaffolds become buried in collagen type II—provide relevant information regarding the viability of the composite scaffolds used and the prospective applications of the proposed approach. In fact, the advanced composite scaffold developed, together with the conditioned medium functionalization, constitutes a biomimetic stem cell niche with clear potential, not just for tendon and ligament repair, but also for cartilage and endochondral bone formation and regeneration strategies. |
| Databáze: | OpenAIRE |
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