Immunogenicity studies with haptenated melittin peptides: implication for membrane involvement during the recognition step
| Autor: | Hanspeter Rolli, Conrad H. Schneider, Véronique Curcio‐Vonlanthen |
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| Rok vydání: | 1996 |
| Předmět: |
Guinea Pigs
Immunoblotting Molecular Sequence Data chemical and pharmacologic phenomena Peptide Biology Biochemistry Melittin Cell membrane chemistry.chemical_compound Epitopes Mice Structural Biology Drug Discovery medicine Animals Amino Acid Sequence Molecular Biology Pharmacology chemistry.chemical_classification Mice Inbred BALB C Immunogenicity Organic Chemistry Cell Membrane General Medicine Melitten Dinitrobenzenes Membrane medicine.anatomical_structure Immunization chemistry Immunoglobulin G Molecular Medicine Paratope Female Peptides Hapten Haptens |
| Zdroj: | Journal of peptide science : an official publication of the European Peptide Society. 2(4) |
| ISSN: | 1075-2617 |
| Popis: | Melittin peptides carrying 2,4-dinitro-6-carboxyphenyl (Dncp) haptenic groups regularly evoked anti-hapten IgG responses in mice or guinea pigs when the hapten was C-terminally attached. Single haptens on the N-terminal helix in several positions gave poor or no responses in the early stages but adequate titres after prolonged immunization. Peptides with Dncp at the C-terminus as an invariant feature and a second Dncp in various positions along the peptide chain did not fail to produce adequate responses. The hampering effect is not due to a defect at the T-cell level but involves the recognition step on the B-cell. It is implied that the haptenic interaction with the paratope of the recognizing immunoglob ulin on the B-cell involves the cell membrane in an important way. It is also suggested that late antibody responses should not be overlooked during the development of proteinaceous immunogens for vaccination. |
| Databáze: | OpenAIRE |
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