Rapid metagenomic characterization of a case of imported COVID-19 in Cambodia
| Autor: | Jonathan Sheu, Jennifer A. Bohl, Yi Sengdoeurn, Christina M Tato, Erik A. Karlsson, Seng Heng, Philippe Dussart, Vida Ahyong, Chan Vuthy, Laurence Baril, Michelle Tan, Katarina Kalantar, Sreyngim Lay, Sophana Chea, Jessica E. Manning, Sovann Ly, Veasna Duong, Joseph L. DeRisi |
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| Přispěvatelé: | National Institute of Allergy and Infectious Diseases [Phnom Penh, Cambodia] (NIAID), National Institute of Allergy and Infectious Diseases [Bethesda] (NIAID-NIH), National Institutes of Health [Bethesda] (NIH)-National Institutes of Health [Bethesda] (NIH), Ministry of Health [Phnom Penh], Chan Zuckerberg Initiative [Redwood City, CA] (CZI), Chan Zuckerberg BioHub [San Francisco, CA], Institut Pasteur du Cambodge, Réseau International des Instituts Pasteur (RIIP), Unité de Virologie / Virology Unit [Phnom Penh], Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), The work of Dr. Manning is supported by the Division of Intramural Research at the National Institute of Allergy and Infectious Diseases at the National Institutes of Health and the Bill and Melinda Gates Foundation. The work of Dr. Karlsson is supported, in part, by , by the U.S. Department of Health and Human Services, Office of the Assistant Secretary for Preparedness and Response (Grant No. 1 IDSEP190051-01-00) and through internal funding at Institut Pasteur in Cambodia. |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0303 health sciences
metagenomics Coronavirus disease 2019 (COVID-19) SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) coronavirus Outbreak COVID-19 Computational biology sequencing Genome Article DNA sequencing Deep sequencing 3. Good health 03 medical and health sciences 0302 clinical medicine Geography unbiased metagenomics Metagenomics 030220 oncology & carcinogenesis [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology 030304 developmental biology |
| Zdroj: | bioRxiv |
| Popis: | Rapid production and publication of pathogen genome sequences during emerging disease outbreaks provide crucial public health information. In resource-limited settings, especially near an outbreak epicenter, conventional deep sequencing or bioinformatics are often challenging. Here we successfully used metagenomic next generation sequencing on an iSeq100 Illumina platform paired with an open-source bioinformatics pipeline to quickly characterize Cambodia’s first case of COVID-2019. |
| Databáze: | OpenAIRE |
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