Inhibition of replication of primary HIV-1 isolates in huPBL-NOD/Scid mice by antibodies from HIV-1 infected patients
| Autor: | Katrien Fransen, Guido Vanham, Leo Heyndrickx, Tine Vermoesen, Helen Donners, Filip Van Wanzeele, Beatrijs Vandergucht, Peter Vanlandschoot, Sophia Steyaert, Lieven Verhoye, Geert Leroux-Roels |
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| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Adult
Male HIV Infections Mice SCID Virus Replication Immunoglobulin G Virus Antibodies Mice Mice Inbred NOD Neutralization Tests Virology Animals Humans Aged Pharmacology biology Immunization Passive Middle Aged Viral Load biology.organism_classification CD4 Lymphocyte Count Disease Models Animal Immunization Viral replication Lentivirus Immunology biology.protein HIV-1 Leukocytes Mononuclear Female Viral disease Antibody Viral load |
| Zdroj: | Antiviral research |
| ISSN: | 0166-3542 |
| Popis: | Although a limited number of HIV-infected patients have broadly neutralizing antibodies, it has not been examined whether these antibodies can protect against infection with primary virus in vivo. Here we screened the plasma of 23 HIV-1-infected patients for broadly neutralizing antibodies. Purified antibodies from subjects with broad and more narrow responses were administered to huPBL-NOD/Scid mice that were subsequently challenged with primary viruses of clade A, B and CRF01_AE. Although we observed a lack of correlation between the data from the in vitro neutralization assay and the results from the passive immunization experiments, we report for the first time that antibodies from HIV-infected persons can inhibit replication of primary virus isolates in an animal model. |
| Databáze: | OpenAIRE |
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