Endogenous factor activating Na,K-ATPase induced by blockade of adrenoceptors
| Autor: | Pavel A. Erokhov, Manukhin Bn, Volina Ev |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Male
medicine.medical_specialty Adrenergic receptor Membrane Fluidity Endogeny Biology Biochemistry Phentolamine Postsynaptic potential Internal medicine medicine Animals Rats Wistar Na+/K+-ATPase Adrenergic alpha-Antagonists Pharmacology Molecular mass Tissue Extracts Brain In vitro Rats Enzyme Activation Molecular Weight Endocrinology Mechanism of action Potentiometry Biophysics Isoelectric Focusing Sodium-Potassium-Exchanging ATPase medicine.symptom medicine.drug |
| Zdroj: | Biochemical Pharmacology. 46:125-130 |
| ISSN: | 0006-2952 |
| DOI: | 10.1016/0006-2952(93)90356-2 |
| Popis: | It was established that the regulatory factor released from the effector cells under the blockade of postsynaptic α- and β-adrenoceptors by phentolamine and propranolol (0.5–1.0 μM) and activating noradrenergic uptake and synthesis [1, 2] increased the activity of Na,K-ATPase of isolated rat organs and partially purified Na, K-ATPase isolated from the rat brain. The regulatory factor is a protein with molecular mass 25–100 kDa, adsorbed on phenylsepharose CL-4B and eluated with 30 mM KC1. It consists of two active components with pI 5.1 and 5.9 which in admixture (1:1) activate Na,K-ATPase by 60%. |
| Databáze: | OpenAIRE |
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