Determination of the release of PEG and HPMC from nelfilcon A daily disposable contact lenses using a novel in vitro eye model

Autor:	Charis Lau, Lakshman N. Subbaraman, Hendrik Walther, Chau-Minh Phan, Donald Riederer, Kathrine Osborn Lorenz, Lyndon Jones, Richard W. Smith
Rok vydání:	2018
Předmět:	Time Factors Biomedical Engineering Biophysics Bioengineering Polyethylene glycol 01 natural sciences Hydrogel Polyethylene Glycol Dimethacrylate Polyethylene Glycols Biomaterials 03 medical and health sciences chemistry.chemical_compound Hypromellose Derivatives 0302 clinical medicine Tandem Mass Spectrometry Liquid chromatography–mass spectrometry PEG ratio Humans Chromatography High Pressure Liquid Chromatography Elution 010401 analytical chemistry technology industry and agriculture Contact Lenses Hydrophilic In vitro 0104 chemical sciences body regions Contact lens Drug Liberation chemistry Tears Drug delivery 030221 ophthalmology & optometry Wetting
Zdroj:	Journal of Biomaterials Science, Polymer Edition. 29:2124-2136
ISSN:	1568-5624 0920-5063
DOI:	10.1080/09205063.2018.1514192
Popis:	The traditional method to measure release of components from CLs is a vial containing a static volume of PBS (phosphate buffered saline). However, this model does not simulate physiologically relevant tear volume and natural tear flow, air exposure, and mechanical rubbing. These factors can significantly impact release kinetics. We have developed an in vitro eye model (OcuFlow) that simulates these parameters. The aim of the study was to measure the release of PEG (polyethylene glycol), and HPMC (hydroxypropyl methylcellulose) from a daily disposable hydrogel contact lens material (nelfilcon A; Dailies AquaComfort PLUS; DACP;) over 24 hrs using the OcuFlow platform. The elution of PEG and HPMC from DACP lenses was analyzed using LCMS (liquid chromatography mass spectrometry). The release of all wetting agents from the lenses followed a burst release pattern, which occurred within the first 1.5 hrs (P 0.05). The release of PEG was greater than that of HPMC (P 0.05). The amount of PEG and HPMC released at any given time was less than 1% of the amount in the blister pack solution. Our results suggest that HPMC and PEG are rapidly released from the CL.
Databáze:	OpenAIRE
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