A cDNA, from Agrotis ipsilon, that encodes the pheromone biosynthesis activating neuropeptide (PBAN) and other FXPRL peptides
| Autor: | Christophe Gadenne, Franck Couillaud, Line Duportets |
|---|---|
| Přispěvatelé: | Université Sciences et Technologies - Bordeaux 1, Unité Mixte de Recherche en Santé Végétale (INRA/ENITA) (UMRSV), Institut National de la Recherche Agronomique (INRA)-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut des Sciences de la Vigne et du Vin (ISVV) |
| Rok vydání: | 1999 |
| Předmět: |
Male
0106 biological sciences DNA Complementary Physiology [SDV]Life Sciences [q-bio] Molecular Sequence Data Prohormone Agrotis ipsilon Saccharomyces cerevisiae Moths 01 natural sciences Biochemistry 03 medical and health sciences Cellular and Molecular Neuroscience Endocrinology Bombyx mori Complementary DNA medicine Animals Amino Acid Sequence RNA Messenger Cloning Molecular Peptide sequence 030304 developmental biology 0303 health sciences Base Sequence Sequence Homology Amino Acid biology Neuropeptides fungi Gene Expression Regulation Developmental LEPIDOPTERE biology.organism_classification Molecular biology INSECTE 010602 entomology Pheromone biosynthesis activating neuropeptide Female Heterologous expression Isoleucine medicine.drug |
| Zdroj: | Peptides Peptides, Elsevier, 1999, 20 (8), pp.899-905. ⟨10.1016/S0196-9781(99)00079-0⟩ |
| ISSN: | 0196-9781 |
| Popis: | International audience; A cDNA encoding the prohormone of the pheromone biosynthesis activating neuropeptide (PBAN) in the moth Agrotis ipsilon was isolated. The cDNA contains 834 nucleotides, coding for a 193-amino acid protein that exhibits 89% identity with PBAN prohormones of other moths. The prohormone contains five potential peptides belonging to the FXPRL family. The peptide corresponding to the Bombyx mori diapause hormone exhibits an extra residue, and the C-terminal leucine is replaced by an isoleucine, introducing a new type of variability in this family of peptides. Northern blot analysis revealed expression in suboesophagal ganglion complexes. Constitutive heterologous expression of Agi-PBAN cDNA in yeast, using three different antibodies, did not produce PBAN-immunoreactive material. |
| Databáze: | OpenAIRE |
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