Chemical Hypoxia-induced Glucose Transporter-4 Translocation in Neonatal Rat Cardiomyocytes
| Autor: | Jia Luo, Jian Hu, Fu Guan, Bo Yu, Ding-yin Zeng, Guo-xian Qi |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
medicine.medical_specialty
medicine.medical_treatment Glucose uptake Respiratory chain AMP-Activated Protein Kinases Protein Serine-Threonine Kinases Carbohydrate metabolism Biology AMP-Activated Protein Kinase Kinases AMP-activated protein kinase Multienzyme Complexes Internal medicine medicine Animals Hypoglycemic Agents Myocytes Cardiac Rats Wistar Sodium Azide Protein Kinase Inhibitors Glucose Transporter Type 4 Insulin Glucose transporter AMPK General Medicine Ribonucleotides Hypoxia (medical) Aminoimidazole Carboxamide Cell Hypoxia Rats Protein Transport Endocrinology Animals Newborn biology.protein medicine.symptom Protein Kinases |
| Zdroj: | Archives of Medical Research. 39:52-60 |
| ISSN: | 0188-4409 |
| Popis: | Background AMP-activated protein kinase (AMPK) activation plays an essential role in glucose metabolism of the heart. This study aimed at investigating whether AMPK was involved in glucose transporter-4 (GLUT-4) translocation induced by azide-induced chemical hypoxia in primary cultured neonatal rat cardiomyocytes. Methods With or without adenine 9-β-D-arabinofuranoside (ara A, AMPK inbibitor) preincubation, primary cultured rat cardiomyocytes were randomized to several groups as incubated with azide (the respiratory chain inhibitor), insulin, or 5-aminoimidazole-4-carboxyamide-1-β-D-ribofuranoside (AICAR, an AMPK activator). Glucose uptake was measured through γ-scintillation and GLUT-4 protein was detected by Western blot for each group. Results Azide-induced chemical hypoxia and AICAR both increased glucose uptake and GLUT-4 translocation in cardiomyocytes, and AICAR had an additive effect on insulin action. Ara A decreased AICAR- and azide-induced glucose uptake and GLUT-4 translocation but did not affect basal or insulin-stimulated glucose uptake. Conclusions Azide-induced chemical hypoxia increased glucose uptake and GLUT-4 translocation in neonatal rat cardiomyocytes through a mechanism that at least was partially mediated by AMPK activation. |
| Databáze: | OpenAIRE |
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