Fatal Amanita muscaria poisoning in a dog confirmed by PCR identification of mushrooms
| Autor: | Michael S. Filigenzi, Hung K Doan, Cynthia L. Gaskill, Megan C Romano, Uneeda K. Bryant, Robert H. Poppenga |
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| Rok vydání: | 2019 |
| Předmět: |
Male
animal structures 040301 veterinary sciences Amanita Kentucky Mushroom Poisoning Urine Polymerase Chain Reaction Mass Spectrometry 030308 mycology & parasitology 0403 veterinary science 03 medical and health sciences chemistry.chemical_compound Dogs Fatal Outcome medicine Animals Dog Diseases Mushroom poisoning Ibotenic Acid 0303 health sciences Mushroom General Veterinary Traditional medicine biology business.industry Muscimol fungi food and beverages 04 agricultural and veterinary sciences medicine.disease biology.organism_classification Gastrointestinal Contents Diarrhea chemistry nervous system Vomiting Identification (biology) medicine.symptom business Brief Communications Ibotenic acid Amanita muscaria psychological phenomena and processes Chromatography Liquid |
| Zdroj: | Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. 31(3) |
| ISSN: | 1943-4936 |
| Popis: | Diagnosing mushroom poisoning in dogs can be difficult and often includes identification of suspect mushrooms. Visual identification may be hindered by mastication, oral medications, or poor quality of environmental mushroom samples. Other analytical techniques may thus be necessary to aid in mushroom identification. A 5-y-old neutered male Labrador Retriever dog developed acute onset of vomiting, diarrhea, tremors, seizures, and somnolence. The dog was treated at a veterinary clinic and was briefly stabilized, but died during transport to an emergency clinic. On postmortem examination at the University of Kentucky Veterinary Diagnostic Laboratory, the dog’s stomach was full of mushrooms covered with activated charcoal. Mushrooms were damaged, fragmented, and discolored, precluding accurate visual identification. Mushroom pieces were sent to the Department of Plant Pathology at the University of California–Davis for PCR identification; the neurotoxic mushroom Amanita muscaria was identified. A qualitative liquid chromatography–mass spectrometry (LC-MS) method was developed to detect ibotenic acid and muscimol, the toxic compounds present in A. muscaria. Mushrooms, stomach contents, and urine were analyzed by LC-MS; ibotenic acid and muscimol were detected in all samples. Because identification of ingested mushrooms is sometimes necessary to confirm mushroom poisoning, PCR can identify ingested mushrooms when visual identification is unreliable. |
| Databáze: | OpenAIRE |
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