A four-year survey (2011–2014) of West Nile virus infection in humans, mosquitoes and birds, including the 2012 meningoencephalitis outbreak in Tunisia
| Autor: | Meriadeg Ar Gouilh, Chawki Loussaief, Najet Dimassi, Jordi Serra-Cobo, Lamjed Boughzala, Maha Mastouri, Badereddine Mechri, Mohamed Chakroun, Abir Monastiri, Ana Vázquez-González, Mahjoub Aouni |
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| Přispěvatelé: | Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives [Monastir] (LR99ES27), Faculté de Pharmacie [Monastir] (FPHM)-Université de Monastir - University of Monastir (UM), Instituto de Salud Carlos III [Madrid] (ISC), CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN), Groupe de Recherche sur l'Adaptation Microbienne (GRAM 2.0), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Université de Caen Normandie (UNICAEN), Normandie Université (NU), Département Infection et Epidémiologie - Department of Infection and Epidemiology, Institut Pasteur [Paris], CHU Fattouma Bourguiba [Monastir] (HFB), Institut Supérieur de Biotechnologie de Monastir (ISBM), Université de Monastir - University of Monastir (UM), Ministère de l’Agriculture, des Ressources Hydrauliques et de la Pêche Maritime [Tunisie], Department of Animal Biology (Institute for Research on Biodiversity (IRBio)), University of Barcelona, Abir Monastiri received a fellowship grant from the Ministry of Higher Education and Scientific Research (Tunisia). Funding for this work was provided by the Ministry of Higher Education and Scientific Research (Tunisia)., We gratefully thank all hospital physicians of the Neurological, Pediatric, Emergency and Neurosurgery Departments and the laboratory personnel in the Microbiology Department at the Fattouma Bourguiba University Hospital of Monastir (Tunisia) for their help in obtaining patient clinical samples and laboratory data, the Ministry of Agriculture (Tunisia) and the Regional Office of Agriculture Development (Monastir, Tunisia) for providing field study permissions, mosquito traps and avian samples within the framework of national passive epidemiological surveillance of avian influenza and West Nile virus in wild birds in Tunisia, and all technicians and staff at the Genomics Unit, Centros Cientificos y Tecnologicos UB (CCiTUB) at the Parc Cientific de Barcelona (PCB), University of Barcelona, for technical support., Université de Monastir - University of Monastir (UM)-Faculté de Pharmacie [Monastir] (FPHM), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Institut Pasteur [Paris] (IP) |
| Rok vydání: | 2018 |
| Předmět: |
Male
Epidemiology MESH: Meningoencephalitis/virology MESH: Tunisia/epidemiology Disease Outbreaks law.invention 0403 veterinary science 0302 clinical medicine MESH: Meningoencephalitis/blood Meningoencephalitis [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases MESH: West Nile virus/isolation & purification MESH: Child Drug Discovery MESH: Animals Child MESH: Phylogeny Phylogeny [SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases MESH: Middle Aged virus diseases MESH: Bird Diseases/blood General Medicine MESH: Infant Virus 3. Good health MESH: Young Adult Child Preschool West Nile virus Tunisia Immunology MESH: Mosquito Vectors/virology Microbiology Article 03 medical and health sciences MESH: West Nile Fever/epidemiology Culex pipiens Humans MESH: Cerebrospinal Fluid/virology Epidemiologia Aged MESH: Adolescent MESH: Humans MESH: West Nile Fever/veterinary MESH: Child Preschool Infant Outbreak MESH: Adult MESH: Meningoencephalitis/epidemiology medicine.disease Virology nervous system diseases MESH: Bird Diseases/epidemiology Vector (epidemiology) Parasitology MESH: Female West Nile Fever Tunísia MESH: Meningoencephalitis/veterinary MESH: West Nile virus/physiology animal diseases viruses MESH: West Nile Fever/virology law Waterfowl MESH: Disease Outbreaks Polymerase chain reaction Cerebrospinal Fluid MESH: Aged 2. Zero hunger Transmission (medicine) 04 agricultural and veterinary sciences Middle Aged Culex Infectious Diseases Viruses MESH: Birds Female geographic locations MESH: Bird Diseases/virology Adult Adolescent 040301 veterinary sciences 030231 tropical medicine Mosquito Vectors Biology Birds MESH: Culex/virology Young Adult medicine Animals MESH: West Nile virus/classification Bird Diseases biology.organism_classification MESH: West Nile Fever/blood MESH: Male [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie MESH: West Nile virus/genetics |
| Zdroj: | Emerging microbes & infections Emerging microbes & infections, Earliest : Springer-Nature ; Latest : Taylor & Francis, 2019, 7, pp.28. ⟨10.1038/s41426-018-0028-y⟩ Emerging Microbes & Infections Repisalud Instituto de Salud Carlos III (ISCIII) Dipòsit Digital de la UB Universidad de Barcelona Emerging microbes & infections, 2019, 7, pp.28. ⟨10.1038/s41426-018-0028-y⟩ |
| ISSN: | 2222-1751 |
| DOI: | 10.1038/s41426-018-0028-y |
| Popis: | A West Nile virus (WNV) outbreak occurred in Tunisia between mid-July and December 2012. To assess the epidemiological features of the WNV transmission cycle, human cerebrospinal fluid samples from patients with suspected cases (n = 79), Culex pipiens mosquitoes (n = 583) and serum specimens from domestic and migratory birds (n = 70) were collected for 4 years (2011-2014) in the Tunisian Sahel region. Viral testing was performed by polymerase chain reaction (PCR). The WNV genome was detected in 7 patients (8.8%), 4 Culex pipiens pools, and a domestic mallard (Anas platyrhynchos). All PCR-positive samples were from the Monastir region. Phylogenetic analysis revealed that two different WNV strain groups circulated, and isolates from the reservoir (bird), vector (Culex pipiens), and dead-end hosts (humans) were closely related. The Monastir region is a hot-spot for WNV infection, and the reiterative presence of WNV over the years has increased the risk of viral reemergence in Tunisia, which highlights the need for more enhanced and effective WNV surveillance in humans with public awareness campaigns strengthened by monitoring mosquitoes and maintaining avian surveillance for early detection of WNV circulation. We gratefully thank all hospital physicians of the Neurological, Pediatric, Emergency and Neurosurgery Departments and the laboratory personnel in the Microbiology Department at the Fattouma Bourguiba University Hospital of Monastir (Tunisia) for their help in obtaining patient clinical samples and laboratory data; the Ministry of Agriculture (Tunisia) and the Regional Office of Agriculture Development (Monastir, Tunisia) for providing field study permissions, mosquito traps and avian samples within the framework of national passive epidemiological surveillance of avian influenza and West Nile virus in wild birds in Tunisia; and all technicians and staff at the Genomics Unit, Centros Cientificos y Tecnologicos UB (CCiTUB) at the Parc Cientific de Barcelona (PCB), University of Barcelona, for technical support. Abir Monastiri received a fellowship grant from the Ministry of Higher Education and Scientific Research (Tunisia). Funding for this work was provided by the Ministry of Higher Education and Scientific Research (Tunisia). Sí |
| Databáze: | OpenAIRE |
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