Serum Apoptosis Markers in Acute Liver Failure: A Pilot Study
| Autor: | K. Rajender Reddy, Anna E. Rutherford, Edwyn M Harrison, Adrian Reuben, April R. Gorman, David G. Forcione, Wenyu Lin, Linda S. Hynan, Raymond T. Chung, Jason T. Blackard, Carolina B. De Sa Borges, William M. Le, Obaid S. Shaikh |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Adult
Male medicine.medical_specialty Adolescent Hepatitis C virus Apoptosis Enzyme-Linked Immunosorbent Assay Pilot Projects medicine.disease_cause Severity of Illness Index Gastroenterology Antigen Interferon Internal medicine Humans Medicine fas Receptor Interleukin 6 Aged Aged 80 and over Liver injury Hepatology biology Hepatocyte Growth Factor Interleukin-6 Tumor Necrosis Factor-alpha business.industry digestive oral and skin physiology Liver Failure Acute Middle Aged Prognosis medicine.disease Immunohistochemistry Liver Immunology biology.protein Female Hepatocyte growth factor Tumor necrosis factor alpha business Biomarkers Follow-Up Studies medicine.drug |
| Zdroj: | Clinical Gastroenterology and Hepatology. 5:1477-1483 |
| ISSN: | 1542-3565 |
| DOI: | 10.1016/j.cgh.2007.08.007 |
| Popis: | Background & Aims: We sought to determine whether circulating apoptotic markers are altered in acute liver failure (ALF), differ with etiology, or predict clinical outcome in this condition. Methods: Serum levels of soluble Fas (sFas), tumor necrosis factor–alpha (TNF-), hepatocyte growth factor (HGF), and interleukin-6 (IL-6) were measured in 67 acute liver failure patients, as well as controls. In a subset of the groups, we measured serum M-30 antigen, an exposed neoepitope from caspase cleavage. We also assessed M-30 immunoreactivity in liver tissue of ALF patients and controls. Results: Median levels for TNF-, HGF, IL-6, and M-30 antigen were at least 10-fold greater in ALF than in hepatitis C virus or normal controls (P < .0001). Median day 1 sFas, day 3 sFas, and day 1 HGF levels varied according to etiology of acute liver failure (P .004, P .011, and P .019, respectively), with values for druginduced liver injury and acetaminophen-related ALF higher than other etiologies. Median M-30 antigen levels were significantly higher in patients who were transplanted and/or died (2183 U/L) than spontaneous survivors (1004 U/L) (P .026). M-30 immunoreactivity in liver tissue was significantly greater in ALF patients than HCV controls (P .004). Conclusions: TNF-, HGF, IL-6, and M-30 antigen were significantly elevated in ALF. High levels of sFas and HGF might help to confirm a diagnosis of druginduced liver injury or acetaminophen-related ALF. Higher levels of M-30 antigen are associated with poor clinical outcomes in ALF. |
| Databáze: | OpenAIRE |
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