Entry of Oncolytic Herpes Simplex Virus into Human Squamous Cell Carcinoma Cells by Ultrasound
| Autor: | Soichi Iwai, Shusuke Okunaga, Masakagu Hamada, Yoshiaki Yura, Tomoaki Imai, Noritoshi Meshii, Ayako Takasu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Viral Plaque Assay
squamous cell carcinoma Pathology medicine.medical_specialty Cell microbubble lcsh:QR1-502 Herpesvirus 1 Human Biology medicine.disease_cause Models Biological Article lcsh:Microbiology oncolytic HSV-1 ultrasound Cell membrane Viral entry Cell Line Tumor Virology medicine Humans Ultrasonography Oncolytic Virotherapy business.industry Ultrasound Epithelial Cells Virus Internalization Oncolytic virus Infectious Diseases Herpes simplex virus medicine.anatomical_structure Cell culture Carcinoma Squamous Cell Microscopy Electron Scanning Cancer research business |
| Zdroj: | Viruses, Vol 7, Iss 10, Pp 5610-5618 (2015) Viruses Viruses; Volume 7; Issue 10; Pages: 5610-5618 Volume 7 Issue 10 Pages 5610-5618 |
| ISSN: | 1999-4915 |
| Popis: | Low-intensity ultrasound is a useful method to introduce materials into cells due to the transient formation of micropores, called sonoporations, on the cell membrane. Whether oncolytic herpes simplex virus type 1 (HSV-1) can be introduced into oral squamous cell carcinoma (SCC) cells through membrane pores remains undetermined. Human SCC cell line SAS and oncolytic HSV-1 RH2, which was deficient in the γ134.5 gene and fusogenic, were used. Cells were exposed to ultrasound in the presence or absence of microbubbles. The increase of virus entry was estimated by plaque numbers. Viral infection was hardly established without the adsorption step, but plaque number was increased by the exposure of HSV-1-inoculated cells to ultrasound. Plaque number was also increased even if SAS cells were exposed to ultrasound and inoculated with RH2 without the adsorption step. This effect was abolished when the interval from ultrasound exposure to virus inoculation was prolonged. Scanning electron microscopy revealed depressed spots on the cell surface after exposure to ultrasound. These results suggest that oncolytic HSV-1 RH2 can be introduced into SAS cells through ultrasound-mediated pores of the cell membrane that are resealed after an interval. |
| Databáze: | OpenAIRE |
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