The assessment of post-vasectomy pain in mice using behaviour and the Mouse Grimace Scale

Autor: Kristel Klaus, Matthew C. Leach, Susana G. Sotocinal, Paul A. Flecknell, Amy L. Miller, Maud Scotto di Perrotolo
Rok vydání: 2011
Předmět:
Research design
Male
Mouse
Thiazines
lcsh:Medicine
Sodium Chloride
Meloxicam
0403 veterinary science
Mice
0302 clinical medicine
Postoperative Period
lcsh:Science
Pain Measurement
Animal Management
Analgesics
Pain
Postoperative
Multidisciplinary
Animal Welfare (journal)
Behavior
Animal
Vasectomy
Agriculture
04 agricultural and veterinary sciences
Animal Models
Bupivacaine
Facial Expression
Neurology
Background current
Research Design
Scale (social sciences)
Models
Animal
Medicine
Research Article
medicine.medical_specialty
040301 veterinary sciences
Cognitive Neuroscience
Pain
Animal Welfare
03 medical and health sciences
Physical medicine and rehabilitation
Model Organisms
Post-vasectomy pain
medicine
Animals
Humans
Pain Management
Biology
Facial expression
business.industry
lcsh:R
Surgery
Thiazoles
Pain scoring
lcsh:Q
Veterinary Science
Analgesia
business
030217 neurology & neurosurgery
Neuroscience
Zdroj: PLoS ONE
PLoS ONE, Vol 7, Iss 4, p e35656 (2012)
ISSN: 1932-6203
Popis: Background Current behaviour-based pain assessments for laboratory rodents have significant limitations. Assessment of facial expression changes, as a novel means of pain scoring, may overcome some of these limitations. The Mouse Grimace Scale appears to offer a means of assessing post-operative pain in mice that is as effective as manual behavioural-based scoring, without the limitations of such schemes. Effective assessment of post-operative pain is not only critical for animal welfare, but also the validity of science using animal models. Methodology/Principal Findings This study compared changes in behaviour assessed using both an automated system (“HomeCageScan”) and using manual analysis with changes in facial expressions assessed using the Mouse Grimace Scale (MGS). Mice (n = 6/group) were assessed before and after surgery (scrotal approach vasectomy) and either received saline, meloxicam or bupivacaine. Both the MGS and manual scoring of pain behaviours identified clear differences between the pre and post surgery periods and between those animals receiving analgesia (20 mg/kg meloxicam or 5 mg/kg bupivacaine) or saline post-operatively. Both of these assessments were highly correlated with those showing high MGS scores also exhibiting high frequencies of pain behaviours. Automated behavioural analysis in contrast was only able to detect differences between the pre and post surgery periods. Conclusions In conclusion, both the Mouse Grimace Scale and manual scoring of pain behaviours are assessing the presence of post-surgical pain, whereas automated behavioural analysis could be detecting surgical stress and/or post-surgical pain. This study suggests that the Mouse Grimace Scale could prove to be a quick and easy means of assessing post-surgical pain, and the efficacy of analgesic treatment in mice that overcomes some of the limitations of behaviour-based assessment schemes.
Databáze: OpenAIRE
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