Immobilization of yeast cell walls with surface displayed laccase from Streptomyces cyaneus within dopamine-alginate beads for dye decolorization
| Autor: | Nikolina Popović, Maja Mladenovic, Raluca Ostafe, Olivera Prodanović, Rainer Fischer, Karla Ilić Đurđić, Selin Ece, Dunja Pržulj, Radivoje Prodanovic |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Lysis
Immobilized enzyme Alginates Dopamine 02 engineering and technology Biochemistry Cell wall 03 medical and health sciences chemistry.chemical_compound Cell Wall Structural Biology Coloring Agents Molecular Biology 030304 developmental biology Laccase 0303 health sciences Periodate General Medicine 021001 nanoscience & nanotechnology Streptomyces Yeast Amido black 10B chemistry Amido Black 0210 nano-technology Yeast surface display Nuclear chemistry |
| Zdroj: | International Journal of Biological Macromolecules |
| Popis: | High amounts of toxic textile dyes are released into the environment due to coloring and wastewaters treatment processes' inefficiency. To remove dyes from the environment and wastewaters, researchers focused on applying immobilized enzymes due to mild reaction conditions and enzyme nontoxicity. Laccases are oxidases with wide substrate specificity, capable of degradation of many different dye types. Laccase from Streptomyces cyaneus was expressed on the surface of Saccharomyces cerevisiae EBY100 cells. The specific activity of surface-displayed laccase was increased by toluene-induced lysis to 3.1 U/g of cell walls. For cell wall laccase immobilization within hydrogel beads, alginate was modified by dopamine using periodate oxidation and reductive amination and characterized by UV–Vis, FTIR, and NMR spectroscopy. Cell wall laccase was immobilized within alginate and dopamine-alginate beads additionally cross-linked by oxygen and laccase. The immobilized enzyme's specific activity was two times higher using dopamine-alginate compared to native alginate beads, and immobilization yield increased 16 times. Cell wall laccase immobilized within dopamine-alginate beads decolorized Amido Black 10B, Reactive Black 5, Evans Blue, and Remazol Brilliant Blue with 100% efficiency and after ten rounds of multiple-use retained decolorization efficiency of 90% with Evans Blue and 61% with Amido Black. This is the peer-reviewed version of the article: Popović, N.; Pržulj, D.; Mladenović, M.; Prodanović, O.; Ece, S.; Ilić Đurđić, K.; Ostafe, R.; Fischer, R.; Prodanović, R. Immobilization of Yeast Cell Walls with Surface Displayed Laccase from Streptomyces Cyaneus within Dopamine-Alginate Beads for Dye Decolorization. International Journal of Biological Macromolecules 2021, 181, 1072–1080. [https://doi.org/10.1016/j.ijbiomac.2021.04.115]. Supplementary material: [https://cherry.chem.bg.ac.rs/handle/123456789/4405] |
| Databáze: | OpenAIRE |
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