Comparison Actin- and Glass-Supported Phospholipid Bilayer Diffusion Coefficients
| Autor: | Sharon Ashworth, Ryan Dawes, David J. Neivandt, Edward S. Allgeyer, Sarah M. Sterling |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
0303 health sciences
Membranes Chemistry Biophysics Analytical chemistry Substrate (chemistry) Fluorescence correlation spectroscopy Biological membrane 02 engineering and technology 021001 nanoscience & nanotechnology Polar membrane 03 medical and health sciences Membrane Membrane fluidity lipids (amino acids peptides and proteins) Lipid bilayer phase behavior 0210 nano-technology Lipid bilayer 030304 developmental biology |
| Popis: | The formation of biomimetic lipid membranes has the potential to provide insights into cellular lipid membrane dynamics. The construction of such membranes necessitates not only the utilization of appropriate lipids, but also physiologically relevant substrate/support materials. The substrate materials employed have been shown to have demonstrable effects on the behavior of the overlying lipid membrane, and thus must be studied before use as a model cushion support. To our knowledge, we report the formation and investigation of a novel actin protein-supported lipid membrane. Specifically, inner leaflet lateral mobility of globular actin-supported DMPC (1,2-dimyristoyl-sn-glycero-3-phosphocholine) bilayers, deposited via the Langmuir-Blodgett/Langmuir Schaefer methodology, was investigated by z-scan fluorescence correlation spectroscopy across a temperature range of 20–44°C. The actin substrate was found to decrease the diffusion coefficient when compared to an identical membrane supported on glass. The depression of the diffusion coefficient occurred across all measured temperatures. These results indicated that the actin substrate exerted a direct effect on the fluidity of the lipid membrane and highlighted the fact that the choice of substrate/support is critical in studies of model lipid membranes. |
| Databáze: | OpenAIRE |
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