Detection of chlamydia infection within human testicular biopsies
| Autor: | Kristofor Bogoevski, Alison J. Carey, Anusch Yazdani, Robert I McLachlan, Eileen A. McLaughlin, Luk Rombauts, Emily R Bryan, Crystal Chang, Darren J Katz, Kenneth W. Beagley, Logan K Trim, Charles W. Armitage, Michelle L. Giles |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
Sperm Retrieval Biopsy Physiology Chlamydia trachomatis medicine.disease_cause male infertility law.invention Male infertility 0302 clinical medicine testicular infection law Testis Andrology Asymptomatic Infections Polymerase chain reaction Azoospermia media_common 0303 health sciences 030219 obstetrics & reproductive medicine Chlamydia medicine.diagnostic_test Rehabilitation Obstetrics and Gynecology 3. Good health Original Article medicine.symptom DNA Bacterial medicine.medical_specialty media_common.quotation_subject Urology MEDLINE Context (language use) Fertility Asymptomatic 03 medical and health sciences Internal medicine medicine Humans 030304 developmental biology business.industry Chlamydia-specific antibodies Chlamydia Infections medicine.disease Cross-Sectional Studies Reproductive Medicine STI business |
| Zdroj: | Human Reproduction (Oxford, England) |
| ISSN: | 1460-2350 0268-1161 |
| Popis: | STUDY QUESTIONCan Chlamydia be found in the testes of infertile men?SUMMARY ANSWERChlamydia can be found in 16.7% of fresh testicular biopsies and 45.3% of fixed testicular biopsies taken from a selection of infertile men.WHAT IS KNOWN ALREADYMale chlamydial infection has been understudied despite male and female infections occurring at similar rates. This is particularly true of asymptomatic infections, which occur in 50% of cases. Chlamydial infection has also been associated with increased sperm DNA damage and reduced male fertility.STUDY DESIGN, SIZE, DURATIONWe collected diagnostic (fixed, n = 100) and therapeutic (fresh, n = 18) human testicular biopsies during sperm recovery procedures from moderately to severely infertile men in a cross-sectional approach to sampling.PARTICIPANTS/MATERIALS, SETTING, METHODSThe diagnostic and therapeutic biopsies were tested for Chlamydia-specific DNA and protein, using real-time PCR and immunohistochemical approaches, respectively. Serum samples matched to the fresh biopsies were also assayed for the presence of Chlamydia-specific antibodies using immunoblotting techniques.MAIN RESULTS AND THE ROLE OF CHANCEChlamydial major outer membrane protein was detected in fixed biopsies at a rate of 45.3%. This was confirmed by detection of chlamydial DNA and TC0500 protein (replication marker). C. trachomatis DNA was detected in fresh biopsies at a rate of 16.7%, and the sera from each of these three positive patients contained C. trachomatis-specific antibodies. Overall, C. trachomatis-specific antibodies were detected in 72.2% of the serum samples from the patients providing fresh biopsies, although none of the patients were symptomatic nor had they reported a previous sexually transmitted infection diagnosis including Chlamydia.LIMITATIONS, REASONS FOR CAUTIONNo reproductively healthy male testicular biopsies were tested for the presence of Chlamydia DNA or proteins or Chlamydia-specific antibodies due to the unavailability of these samples.WIDER IMPLICATIONS FOR THE FINDINGSApplication of Chlamydia-specific PCR and immunohistochemistry in this human male infertility context of testicular biopsies reveals evidence of a high prevalence of previously unrecognised infection, which may potentially have a pathogenic role in spermatogenic failure.STUDY FUNDING/COMPETING INTEREST(S)Funding for this project was provided by the Australian NHMRC under project grant number APP1062198. We also acknowledge assistance from the Monash IVF Group and Queensland Fertility Group in the collection of fresh biopsies, and the Monash Health and co-author McLachlan (declared equity interest) in retrieval and sectioning of fixed biopsies. E.M. declares an equity interest in the study due to financing of fixed biopsy sectioning. All other authors declare no conflicts of interest.TRIAL REGISTRATION NUMBERN/A |
| Databáze: | OpenAIRE |
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