Biological Role of Paenilarvins, Iturin-Like Lipopeptide Secondary Metabolites Produced by the Honey Bee Pathogen Paenibacillus larvae
| Autor: | Julia Ebeling, Roderich D. Süssmuth, Gillian Hertlein, Marlene Seiffert, Sebastian Gensel, Anja Kuthning, Ranko Skobalj, Eva Garcia-Gonzalez, Elke Genersch |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Life Cycles genetic structures Cytotoxicity lcsh:Medicine Pathogenesis Pathology and Laboratory Medicine Toxicology Virulence factor chemistry.chemical_compound Mice Larvae Medicine and Health Sciences Bioassay lcsh:Science Pathogen Multidisciplinary Cytotoxicity Assay Lipopeptide Bees Insects Larva Pathogens Honey Bees Research Article American foulbrood animal structures Arthropoda Virulence Factors 030106 microbiology Virulence Biology Microbiology Cell Line 03 medical and health sciences Lipopeptides parasitic diseases Animals Nutrition Paenibacillus larvae fungi lcsh:R Organisms Biology and Life Sciences Midgut Honey bee Invertebrates Hymenoptera Diet 030104 developmental biology chemistry lcsh:Q human activities Developmental Biology |
| Zdroj: | PLoS ONE, Vol 11, Iss 10, p e0164656 (2016) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | The Gram-positive bacterium Paenibacillus larvae (P. larvae) is the causative agent of a deadly honey bee brood disease called American Foulbrood (AFB). AFB is a notifiable epizootic in most countries and, hence, P. larvae is of considerable relevance for veterinarians and apiculturists alike. Over the last decade, much progress has been made in the understanding of the (patho)biology of P. larvae. Recently, several non-ribosomally produced peptides (NRP) and peptide/polyketide (NRP/PK) hybrids produced by P. larvae were identified. Among these NRPs were iturin-like lipopeptides, the paenilarvins A-C. Iturins are known to exhibit strong anti-fungal activity; for some iturins, cytotoxic activity towards mammalian erythrocytes and human cancer cell lines are described. We here present our results on the analysis of the natural function of the paenilarvins during pathogenesis of P. larvae infections. We demonstrated production of paenilarvins in infected larvae. However, we could neither demonstrate cytotoxicity of paenilarvins towards cultured insect cells nor towards larvae in feeding assays. Accordingly, exposure bioassays performed with larvae infected by wild-type P. larvae and a knockout mutant of P. larvae lacking production of paenilarvins did not substantiate a role for the paenilarvins as virulence factor. Further experiments are necessary to analyze the relevance of the paenilarvins' anti-fungal activity for P. larvae infections in the presence of fungal competitors in the larval midgut or cadaver. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|