Cell-free DNA promotes malignant transformation in non-tumor cells

Autor: Ana Carolina Laus, Leandro M. Colli, Natássia Caroline Resende Corrêa, Patrícia Tieme Fujimura, Luiz Ricardo Goulart, Carolina Hassibe Thomé, Luciane Sussuchi da Silva, Victor Alexandre Felix Bastos, Leticia Ferro Leal, Rui Manuel Reis, Aline Gomes de Souza, Karina Marangoni, Vivian Alonso Goulart, Izabella C C Ferreira, Paula de Souza Santos, Mário Machado Martins
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Scientific Reports, Vol 10, Iss 1, Pp 1-10 (2020)
Scientific Reports
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
ISSN: 2045-2322
Popis: Cell-free DNA is present in different biological fluids and when released by tumor cells may contribute to pro-tumor events such as malignant transformation of cells adjacent to the tumor and metastasis. Thus, this study analyzed the effect of tumor cell-free DNA, isolated from the blood of prostate cancer patients, on non-tumor prostate cell lines (RWPE-1 and PNT-2). To achieve this, we performed cell-free DNA quantification and characterization assays, evaluation of gene and miRNA expression profiling focused on cancer progression and EMT, and metabolomics by mass spectrometry and cellular migration. The results showed that tumor-free cell DNA was able to alter the gene expression of MMP9 and CD44, alter the expression profile of nine miRNAs, and increased the tryptophan consumption and cell migration rates in non-tumor cells. Therefore, tumor cell-free DNA was capable of altering the receptor cell phenotype, triggering events related to malignant transformation in these cells, and can thus be considered a potential target for cancer diagnosis and therapy.
Databáze: OpenAIRE
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