Chlojaponilactone B from Chloranthus japonicus: Suppression of Inflammatory Responses via Inhibition of the NF-κB Signaling Pathway
| Autor: | Yan-Qiong Guo, Xue Xue, Jing-jun Zhao, Long-Ping Zhu, Qin Liu, Depo Yang, Zhimin Zhao, Sheng Yin |
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| Rok vydání: | 2016 |
| Předmět: |
Lipopolysaccharides
0301 basic medicine Lipopolysaccharide Chloranthus japonicus Transcription Factor RelA Anti-Inflammatory Agents Nitric Oxide Synthase Type II Pharmaceutical Science Biology Nitric Oxide 01 natural sciences Analytical Chemistry Nitric oxide Mice 03 medical and health sciences chemistry.chemical_compound Drug Discovery medicine Animals Phosphorylation Inflammation Pharmacology Molecular Structure Interleukin-6 Tumor Necrosis Factor-alpha 010405 organic chemistry Macrophages Organic Chemistry NF-kappa B medicine.disease 0104 chemical sciences Cell biology IκBα 030104 developmental biology Complementary and alternative medicine chemistry Biochemistry Cyclooxygenase 2 Cytokines Molecular Medicine Inflammation Mediators Signal transduction Sesquiterpenes Infiltration (medical) Signal Transduction |
| Zdroj: | Journal of Natural Products. 79:2257-2263 |
| ISSN: | 1520-6025 0163-3864 |
| DOI: | 10.1021/acs.jnatprod.6b00355 |
| Popis: | Bioassay-guided fractionation of an ethanolic extract of Chloranthus japonicus led to the isolation of the known lindenane-type sesquiterpenoid chlojaponilactone B (1). This compound exhibited pronounced inhibition of nitric oxide (NO) production in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages. Further anti-inflammatory assays showed that 1 suppressed the levels of some key inflammation mediators, such as iNOS, TNF-α, and IL-6, in a dose-dependent manner, and reduced the ear thickness and neutrophil infiltration in 12-O-tetradecanoylphorbol-13-acetate (TPA)-stimulated mice. A mechanistic study revealed that compound 1 exerted its anti-inflammatory effects via the suppression of the NF-κB signaling pathway, which inhibited NF-κB-dependent transcriptional activity, IκBα phosphorylation, and p65 nuclear translocation. In contrast, chlojaponilactone B (1) was found to exert little influence on the MAPK signaling pathway. |
| Databáze: | OpenAIRE |
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