Over-Expression of Rose RrLAZY1 Negatively Regulates the Branch Angle of Transgenic Arabidopsis Inflorescence
| Autor: | Dan Li, Mingyuan Zhao, Xiaoyan Yu, Lanyong Zhao, Zongda Xu, Xu Han |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
over-expression
QH301-705.5 Arabidopsis Rosa Catalysis Article Inorganic Chemistry RrLAZY1 branch angle Rosa rugosa protein interaction Protein Domains Gene Expression Regulation Plant Two-Hybrid System Techniques Physical and Theoretical Chemistry Biology (General) Cloning Molecular Inflorescence Molecular Biology QD1-999 Spectroscopy Plant Proteins Plant Stems Organic Chemistry Cell Membrane Membrane Proteins General Medicine Sequence Analysis DNA Plants Genetically Modified Computer Science Applications Chemistry Plant Shoots |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 22, Iss 13664, p 13664 (2021) International Journal of Molecular Sciences; Volume 22; Issue 24; Pages: 13664 |
| ISSN: | 1422-0067 |
| Popis: | Branch angle is a key shoot architecture trait that strongly influences the ornamental and economic value of garden plants. However, the mechanism underlying the control of branch angle, an important aspect of tree architecture, is far from clear in roses. In the present study, we isolated the RrLAZY1 gene from the stems of Rosa rugosa ‘Zilong wochi’. Sequence analysis showed that the encoded RrLAZY1 protein contained a conserved GΦL (A/T) IGT domain, which belongs to the IGT family. Quantitative real-time PCR (qRT-PCR) analyses revealed that RrLAZY1 was expressed in all tissues and that expression was highest in the stem. The RrLAZY1 protein was localized in the plasma membrane. Based on a yeast two-hybrid assay and bimolecular fluorescence complementation experiments, the RrLAZY1 protein was found to interact with auxin-related proteins RrIAA16. The over-expression of the RrLAZY1 gene displayed a smaller branch angle in transgenic Arabidopsis inflorescence and resulted in changes in the expression level of genes related to auxin polar transport and signal transduction pathways. This study represents the first systematic analysis of the LAZY1 gene family in R. rugosa. The results of this study will provide a theoretical basis for the improvement of rose plant types and molecular breeding and provide valuable information for studying the regulation mechanism of branch angle in other woody plants. |
| Databáze: | OpenAIRE |
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