Antifatigue effects of Hechong (Tylorrhynchus heterochaetus) through modulation of Nrf2/ARE- mediated antioxidant signaling pathway
| Autor: | Xuchu Zheng, Ziqing Yang, Christudas Sunil, Kuopeng Cui, Yuepeng Su, Baojun Xu |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Antioxidant NF-E2-Related Factor 2 medicine.medical_treatment Blotting Western Toxicology medicine.disease_cause Antioxidants Mice Western blot Internal medicine Animals Outbred Strains medicine Animals Muscle Skeletal Swimming chemistry.chemical_classification Messenger RNA Dose-Response Relationship Drug medicine.diagnostic_test Reverse Transcriptase Polymerase Chain Reaction Tissue Extracts Chemistry Skeletal muscle Polychaeta General Medicine Enzyme Endocrinology medicine.anatomical_structure Liver Mechanism of action Muscle Fatigue Signal transduction medicine.symptom Oxidative stress Signal Transduction Food Science |
| Zdroj: | Food and Chemical Toxicology. 157:112589 |
| ISSN: | 0278-6915 |
| DOI: | 10.1016/j.fct.2021.112589 |
| Popis: | This study was to evaluate the antifatigue effect of T. heterochaetus and explore the underlying mechanism of action. T. heterochaetus extract was treated to mice for 28 days. On the 28th day, after weight loaded swimming test. The levels of antioxidant enzymes and levels of pro- and anti-inflammatory cytokines in the liver and muscles of exercised mice were evaluated. mRNA and protein expression levels of Nrf2, SOD, HO-1, and Keap-1 were evaluated using RT-PCR and western blot analysis. The low (2.70 mg/0.5 ml/20 g) and medium (5.41 mg/0.5 ml/20 g) dose enhanced the activities of antioxidant enzymes like SOD, CAT and GPx in the liver and skeletal muscle thereby enhancing the antifatigue effect. The low and medium doses showed good anti-inflammatory effects by evaluating the levels of pro and anti-inflammatory cytokines such as TNF-α, IL-1β, IL-6, and IL-10 both in the liver and skeletal muscle. Furthermore, RT-PCR and western blot analysis showed increased expression of HO-1, SOD, Nrf2, and decreased expression of Keap-1 gene and proteins in liver and skeletal muscle of T. heterochaetus treated group mice. The current results indicate that T. heterochaetus exert the antifatigue effect through attenuating oxidative stress injury and inflammatory responses through the Nrf2/ARE-mediated signaling pathway. |
| Databáze: | OpenAIRE |
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