Abnormal cell-specific expressions of certain protein kinase C isoenzymes in peripheral mononuclear cells of patients with systemic lupus erythematosus: effect of corticosteroid application
| Autor: | Helga Papp, Zoltán Griger, Gyula Szegedi, Margit Zeher, László Kovács, Kornélia Szücs, Tamás Bíró, Magdolna Aleksza, Edit Bodolay, Emese Kiss, Ilona Kovács, Tünde Csépány, Sándor Sipka, Pál Gergely |
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| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
Adult
Male medicine.medical_specialty T-Lymphocytes Immunology Gene Expression In Vitro Techniques Klinikai orvostudományok Isozyme Peripheral blood mononuclear cell Monocytes Mixed connective tissue disease Adrenal Cortex Hormones In vivo Internal medicine medicine Humans Lupus Erythematosus Systemic skin and connective tissue diseases Protein Kinase C Protein kinase C Mixed Connective Tissue Disease Arachidonic Acid business.industry General Medicine Orvostudományok Middle Aged medicine.disease In vitro Isoenzymes Blot Sjogren's Syndrome Endocrinology Leukocytes Mononuclear Female business Glucocorticoid medicine.drug |
| Popis: | We have studied the expressions of various protein kinase C (PKC) isoenzymes in T cells and monocytes from patients with systemic lupus erythematosus (SLE), in comparison to those of healthy controls and patients with other immunological disorders. As measured by Western blotting, the levels of PKCbeta, delta, eta, epsilon, theta and zeta (but not of PKCalpha) significantly decreased in T cells of SLE patients. In monocytes, however, we observed marked suppressions only in the expressions of PKCdelta, epsilon and zeta but not in the expressions of other PKC isoforms. In vivo corticosteroid application, as well as in vitro steroid treatment of monocytes, elevated the expressions of most isoforms close to normal values; however, the decreased levels of PKCtheta and zeta were not affected by steroid application. These alterations were characteristic to SLE because we could not detect any changes in the PKC levels in mononuclear cells of primary Sjögren's syndrome and mixed connective tissue disease patients. These results suggest that impaired PKC isoenzyme pattern may exist in the T cells and monocytes of SLE patients. Furthermore, the clinically efficient glucocorticoid application in SLE can increase the expression of some members of PKC system. |
| Databáze: | OpenAIRE |
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