Method for the determination of cerebral blood flow in conscious rabbits
| Autor: | Luigia Favalli, Annalinda Rozza Dionigi, F. Piccinini, Carlo Aprile, Enrica Lanza, Angelo Favino |
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| Rok vydání: | 1980 |
| Předmět: |
Time Factors
Slow component Conscious rabbit Microsphere White matter medicine.artery medicine Methods Animals Anesthesia Common carotid artery Radionuclide Imaging Pharmacology business.industry Chemistry Albumin Brain Technetium Anatomy Microspheres medicine.anatomical_structure Carotid Arteries Cerebral blood flow Cerebrovascular Circulation Injections Intravenous Rabbits Internal carotid artery Nuclear medicine business Xenon Radioisotopes |
| Zdroj: | Journal of pharmacological methods. 4(1) |
| ISSN: | 0160-5402 |
| Popis: | A procedure for the determination of cerebral blood flow by the local clearance method after intracarotid injection of 133 Xe in the conscious rabbit is described. The inert radioactive indicator is injected into a permanent nylon catheter equipped with a two-way Gordth's needle inserted into the common carotid artery and filled with heparin, emerging behind the shoulders of the animals. All branches of the homolateral common carotid artery except the internal carotid artery were ligated. Studies of the distribution of colored tracers (dark blue ink) and radioactive tracers ( 99m Tc albumin microspheres) show that the main localization of the injected indicator is within the homolateral hemisphere. Brain to blood partition coefficients of 133 Xe are worked out for rabbit's gray matter (0.576 ± 0.048) and white matter (0.808 ± 0.023). The slope method for first and second component of the wash-out Xenon curve is used for CBF calculations. CBF determinations in 9 normal rabbits result in 84.27 ± 5.59 and 16.69 ± 2.44 ml/min × 100 g tissue, respectively, for the fast and slow component. Significant changes do not occur in serial determinations within 2 hr. |
| Databáze: | OpenAIRE |
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