RP-HPLC and LC–MS–MS determination of a bioactive artefact from Ipomoea pes-caprae extract
| Autor: | Angélica Garcia Couto, Nara Lins Meira Quintão, Camila Assis, Angela Malheiros, Hugo Guilherme Tolentino de Souza, Cechinel Filho, Rivaldo Niero, Cristiane da Silva Barth, Tiago José Bonomini, José Roberto Santin, Camilla Regina de Souza Madeira, Louis P. Sandjo, Tania Mari Bellé Bresolin, Lilian W. Rocha |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Inflammation
Nociception Chromatography biology 010405 organic chemistry Chemistry Extraction (chemistry) lcsh:RS1-441 Ethyl caffeate Ipomoea biology.organism_classification 01 natural sciences High-performance liquid chromatography Ipomoea pes-caprae 0104 chemical sciences Matrix (chemical analysis) Chemometrics lcsh:Pharmacy and materia medica 010404 medicinal & biomolecular chemistry chemistry.chemical_compound Caffeoylquinic acid Biomonitoring General Pharmacology Toxicology and Pharmaceutics |
| Zdroj: | Revista Brasileira de Farmacognosia, Volume: 29, Issue: 5, Pages: 570-577, Published: 09 DEC 2019 Revista Brasileira de Farmacognosia v.29 n.5 2019 Revista Brasileira de Farmacognosia Sociedade Brasileira de Farmacognosia (SBFgnosia) instacron:SBFGNOSIA Revista Brasileira de Farmacognosia, Vol 29, Iss 5, Pp 570-577 (2019) |
| Popis: | Solvents play important and critical role in natural product chemistry and could generate artefacts during the extraction and purification of metabolites from a biological matrix. This study aimed to correlate the chromatographic profile with biological activity of Ipomoea pes-caprae (L.) R. Br., Convolvulaceae, extracts obtained with hydroethanolic extraction. Thus, aerial parts of I. pes-caprae were extracted with different concentration of ethanol (50, 70 and 90°GL) and the obtained extracts were analysed by HPLC–UV. HPLC data were studied employing chemometrics to discriminate the samples. Moreover these samples were further characterized by using UPLC–QTOF/MS data. The extracts were also biomonitored through the paw-oedema and spontaneous nociception induced by trypsin in mice. Different chromatographic profiles were obtained and the exploratory analysis clearly revealed higher level of ethyl caffeate in extracts of lower strength of ethanol (50°GL). This compound was suggested to be an artefact formed by transesterification of caffeoylquinic acid derivatives present in the plant, once it was not observed when other solvents were employed. During the biological assay, only the extract obtained with ethanol 50°GL presented significant inhibition of inflammation (45 ± 9%) and nociception (24 ± 3%). Ethyl caffeate seems to be linked to the anti-inflammatory effect since it reduced 86 ± 5% of paw-oedema induced by trypsin. Artefacts could contribute to the biological activity of herbal preparations and consequently lead to misinterpretation of the results. Keywords: Chemometrics, Biomonitoring, Inflammation, Nociception, Ethyl caffeate |
| Databáze: | OpenAIRE |
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