Interaction of epidermal growth factor receptors with the cytoskeleton is related to receptor clustering
| Autor: | Marcel Spaargaren, Arie J. Verkleij, Nico van Belzen, Johannes Boonstra |
|---|---|
| Přispěvatelé: | Other departments |
| Jazyk: | angličtina |
| Rok vydání: | 1990 |
| Předmět: |
Physiology
Clinical Biochemistry macromolecular substances Cytoplasmic receptor Biology Transfection Structure-Activity Relationship Epidermal growth factor Animals Humans Kinase activity Phosphorylation Receptor Cytoskeleton Epidermal Growth Factor Receptor Aggregation Membrane Proteins Cell Biology Protein-Tyrosine Kinases Recombinant Proteins ErbB Receptors Sphingomyelin Phosphodiesterase Biochemistry Membrane protein Receptor clustering Chromosome Deletion Intracellular |
| Zdroj: | Journal of cellular physiology, 145(2), 365-375. Wiley-Liss Inc. |
| ISSN: | 0021-9541 |
| DOI: | 10.1002/jcp.1041450223 |
| Popis: | Recently it has been established that cytoskeleton-associated epidermal growth factor (EGF) receptors are predominantly of the high-affinity class and that EGF induces a recruitment of low-affinity receptors to the cytoskeleton. The nature of this EGF-induced receptor-cytoskeleton interaction, however, is still unknown. Therefore, we have studied the association of mutated EGF receptors with the cytoskeleton. Receptor deletion mutants lacking almost all intracellular amino acid residues displayed no interaction with the cytoskeleton, demonstrating that the cytoplasmic receptor domain is involved in this interaction. Further analysis revealed that receptor-cytoskeleton interaction is independent of receptor kinase activity and the C-terminal 126 amino acid residues, which include the auto-phosphorylation sites. Furthermore, it is shown that the high-affinity receptor subclass is not essential for association of low-affinity receptors to the cytoskeleton. EGF receptor-cytoskeleton interaction was increased, however, by treatment with sphingomyelinase, an enzyme known to induce membrane protein clustering, indicating that EGF receptor clustering may cause the association to the cytoskeleton. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text