A study on mutation points of GDF9 gene and their association with prolificacy in Egyptian small ruminants
| Autor: | Inas S. Ghaly, Neama I. Ali, Ahmed M. Darwish, Ibrahim Farag, Dalia M. Aboelhassan |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Veterinary medicine Breeding program media_common.quotation_subject QH426-470 Biology Egyptian sheep and goats 03 medical and health sciences GDF9 gene Genotype Genetics Gene markers Gene media_common Research Domestic sheep reproduction 0402 animal and dairy science 04 agricultural and veterinary sciences Fecundity 040201 dairy & animal science Major gene 030104 developmental biology Genetic marker T-ARMS-PCR Prolificacy Reproduction TP248.13-248.65 Biotechnology |
| Zdroj: | Journal of Genetic Engineering & Biotechnology Journal of Genetic Engineering and Biotechnology, Vol 19, Iss 1, Pp 1-11 (2021) |
| ISSN: | 2090-5920 |
| DOI: | 10.1186/s43141-021-00181-8 |
| Popis: | Background Genetic variants of the GDF9 gene were considered to be the potent gene markers for improving fecundity traits in Egyptian sheep and goats. Also, these favorable gene variants could be applied in the breeding program by gene-assisted selection (GAS), aiming towards the potential amelioration of reproduction and production in such small ruminants. The present investigation was designed to evaluate the genetic variants of the GDF9 gene on fecundity traits including the mean number of lambing “MNL” and mean number of twin production “MNTP” of Egyptian sheep and goats. Results This experiment involved 113 mothers, 83 of sheep and 30 of goats, at first, second, third, and fourth parity, and also 26 young females, 12 of sheep and 14 of goats at age of sexual maturation. T-ARMS-PCR analysis was performed on five mutation points (G1, G4, G6, G7, and G8). In sheep, the heterozygous mothers of G4 had significant elevation (P ≤ 0.05) of MNL and MNTP than wild-type homozygous ewes. However, the heterozygous mothers of G1 and G6 gave a reduction of MNL and MNTP as compared to mothers with wild-type genotypes. The ewes of G7 had heterozygous genotype (AG), and the ewes of G8 had wild type (CC). In goat, G4 and G7 were polymorphic, and G1, G6, and G8 were monomorphic type. Based on these findings, it must be selected the young sheep females of heterozygous in G4, and the young goat females of heterozygous in G4 and G7 for participating in a successful breeding program, because they will have potential high fecundity traits. Conclusion The present results confirmed that the genetic variants of the GDF9 gene were considered to be the major gene markers for enhancement of the prolificacy in Egyptian sheep and goats and could be applied in a successful breeding program by gene-assisted selection (GAS) in small ruminants. |
| Databáze: | OpenAIRE |
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