Apoptosis in Cultured Human Fetal Membrane Cells Infected with Influenza Virus
| Autor: | Kunio Ohyama, Bo Yuan, Noboru Uchide, Toshio Yamakawa, Toshio Bessho |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
endocrine system
viruses Orthomyxoviridae Extraembryonic Membranes Pharmaceutical Science Apoptosis DNA Fragmentation Viral Plaque Assay Virus Replication Antiviral Agents Virus Multiplicity of infection Cytopathogenic Effect Viral Pregnancy Fetal membrane Influenza Human Ribavirin medicine Humans Fragmentation (cell biology) Cells Cultured reproductive and urinary physiology Pharmacology L-Lactate Dehydrogenase Amnion biology virus diseases Chorion General Medicine biology.organism_classification Virology medicine.anatomical_structure Influenza A virus embryonic structures DNA fragmentation Female |
| Zdroj: | Biological and Pharmaceutical Bulletin. 25:109-114 |
| ISSN: | 1347-5215 0918-6158 |
| DOI: | 10.1248/bpb.25.109 |
| Popis: | We investigated the induction of apoptosis in cultured human fetal membrane cells infected with influenza virus type A. We found that influenza virus yield in supernatants of primary cultured chorion and amnion cells prepared from human fetal membranes increased 6 h after infection. Chromosomal DNA was fragmented into oligonucleosomes at 48 h after influenza virus infection in chorion cells but not in mock-infected chorion cells, mock-infected amnion cells or influenza virus-infected amnion cells. The DNA fragmentation in influenza virus-infected chorion cells was evident at 24 h after infection and depended on the multiplicity of infection at 48 h. Incubating influenza virus-infected chorion cells with ribavirin, an inhibitor of viral RNA synthesis, reduced the increase in virus yield and simultaneously blocked DNA fragmentation. These results suggest that both chorion and amnion cells become infected with influenza virus, but that influenza virus infection induces apoptosis in chorion, but not typical apoptosis characterized by DNA ladder formation in amnion cells. We further observed that influenza virus replication is associated with the induction of apoptosis. |
| Databáze: | OpenAIRE |
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