Effect of the pH of growth on the survival of Lactobacillus delbrueckii subsp. bulgaricus to stress conditions during spray-drying
| Autor: | Paula Teixeira, Rita Ferreira, Ana Sofia Carvalho, Francisco Amado, Pedro Domingues, Joana Silva, Rui Vitorino, Paul Gibbs |
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| Přispěvatelé: | Veritati - Repositório Institucional da Universidade Católica Portuguesa |
| Rok vydání: | 2005 |
| Předmět: |
Thermotolerance
Spectrometry Mass Electrospray Ionization GroES Protein Blotting Western Spray-drying Storage Biology pH growth conditions Applied Microbiology and Biotechnology Bacterial Proteins Lactobacillus bulgaricus Thermo-tolerance Food Preservation Lactobacillus Heat shock protein Chaperonin 10 Electrophoresis Gel Two-Dimensional HSP70 Heat-Shock Proteins Food science Desiccation Gel electrophoresis Lactobacillus delbrueckii Heat shock proteins Chaperonin 60 General Medicine GroES Hydrogen-Ion Concentration biology.organism_classification GroEL Hsp70 Biochemistry Food Microbiology Lactobacillus delbrueckii subsp. bulgaricus Biotechnology |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| ISSN: | 1365-2672 1364-5072 |
| DOI: | 10.1111/j.1365-2672.2004.02516.x |
| Popis: | Aims: The aim of this study was to optimize survival of Lactobacillus delbrueckii subsp. bulgaricus during spray-drying and subsequent storage through optimizing the pH of growth conditions. Methods and Results: Cell concentrates previously grown without or with pH controlled were spray-dried and stored at 20°C and heat treated at 57°C. Cells grown under noncontrolled pH were more resistant to both drying and heating than cells grown under controlled pH but no significant differences were observed during storage. The intracellular proteins profile of cells grown under both conditions was studied by two-dimensional SDS-polyacrylamide gel electrophoresis. Eight proteins were identified using automated mass spectrometry (MS) and tandem mass spectrometry (MS/MS) data acquisition. Of the identified proteins, only cochaperonin GroES corresponded to a known heat shock protein (HSP). The other proteins identified are proteins involved in glycolysis. For cells grown under noncontrolled pH the expression of the Hsp70, GroES and GroEL, measured by Western blotting, was enhanced. Conclusions: The higher resistance of cells grown under noncontrolled pH correlates with the enhanced production of heat shock proteins. Significance and Impact of the Study: Growth of L. bulgaricus under controlled pH (commonly used by the starter cultures production industry) results in cells more sensitive to stresses frequently encountered by the cells during starter cultures preparation/storage/utilization. |
| Databáze: | OpenAIRE |
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