La taxonomie bactérienne moderne : revue des techniques — application à la caractérisation des bactéries nodulant les légumineuses (BNL)
| Autor: | Philippe de Lajudie, Frédéric Zakhia |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
biology
Plant roots Microorganism Immunology Bacterial taxonomy General Medicine biology.organism_classification Applied Microbiology and Biotechnology Microbiology law.invention molecular characterization prokaryotes law polyphasic taxonomy Botany Genetics Rhizobium Taxonomy (biology) legume nodulating bacteria bacteria Molecular Biology Nomenclature Bacteria Polymerase chain reaction |
| Zdroj: | Canadian Journal of Microbiology. 52:169-181 |
| ISSN: | 1480-3275 0008-4166 |
| Popis: | Taxonomy is the science that studies the relationships between organisms. It comprises classification, nomenclature, and identification. Modern bacterial taxonomy is polyphasic. This means that it is based on several molecular techniques, each one retrieving the information at different cellular levels (proteins, fatty acids, DNA...). The obtained results are combined and analysed to reach a "consensus taxonomy" of a microorganism. Until 1970, a small number of classification techniques were available for microbiologists (mainly phenotypic characterization was performed: a legume species nodulation ability for a Rhizobium, for example). With the development of techniques based on polymerase chain reaction for characterization, the bacterial taxonomy has undergone great changes. In particular, the classification of the legume nodulating bacteria has been repeatedly modified over the last 20 years. We present here a review of the currently used molecular techniques in bacterial characterization, with examples of application of these techniques for the study of the legume nodulating bacteria.Key words: polyphasic taxonomy, molecular characterization, bacteria, prokaryotes, legume nodulating bacteria, Rhizobium. |
| Databáze: | OpenAIRE |
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