Expression, purification, crystallization and preliminary X-ray diffraction analysis of swine leukocyte antigen 2 complexed with a CTL epitope AS64 derived from Asia1 serotype of foot-and-mouth disease virus
| Autor: | Xiao-xin Zhai, Lei Feng, Zong-hui Zhang, Ping Jiang, Zi-bin Li, Xiao Hu, Hua Gao, Feng-shan Gao |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Serotype Models Molecular Protein Folding Swine animal diseases Cell Major histocompatibility complex Serogroup Swine leukocyte antigen 2 Epitope Virus 03 medical and health sciences Epitopes 0302 clinical medicine X-Ray Diffraction medicine Cytotoxic T cell Animals Protein Structure Quaternary lcsh:Veterinary medicine General Veterinary biology Chemistry Foot-and-mouth disease virus Crystal structure fungi Histocompatibility Antigens Class I General Medicine biology.organism_classification Virology CTL 030104 developmental biology medicine.anatomical_structure biology.protein lcsh:SF600-1100 Asia 1 serotype Crystallization 030215 immunology T-Lymphocytes Cytotoxic Research Article |
| Zdroj: | BMC Veterinary Research BMC Veterinary Research, Vol 14, Iss 1, Pp 1-6 (2018) |
| ISSN: | 1746-6148 |
| Popis: | Background Currently, the structural characteristics of the swine major histocompatibility complex (MHC) class I molecule, also named swine leukocyte antigen class I (SLA-I) molecule need to be further clarified. Results A complex of SLA-I constituted by an SLA-2*HB01 molecule with swine β2-microglobulin and a cytotoxic T lymphocyte (CTL) epitope FMDV-AS64 (ALLRSATYY) derived from VP1 protein (residues 64–72) of Asia 1 serotype of foot-and-mouth disease virus (FMDV) was expressed, refolded, purified and crystallized. By preliminary X-ray diffraction analysis, it was shown that the diffraction resolution of the crystal was 2.4 Å and the space group belonged to P212121 with unit cell parameters a = 48.37, b = 97.75, c = 166.163 Å. Conclusion This research will be in favor of illuminating the structural characteristics of an SLA-2 molecule associated with a CTL epitope derived from Asia1 serotype of FMDV. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |