Ex vivo bioluminescence imaging of late gestation ewes following intrauterine inoculation with lux-modified Escherichia coli
| Autor: | Sheryl Rodts-Palenik, Scott T. Willard, Chad K Klauser, Peter L. Ryan, William A. Bennett, Keesla Moulton, R. M. Hopper, David L. Christiansen |
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| Rok vydání: | 2009 |
| Předmět: |
Immunology
medicine.disease_cause Microbiology Obstetric Labor Premature Pregnancy Escherichia coli medicine Animals Humans Immunology and Allergy Bioluminescence imaging Bioluminescence Pregnancy Complications Infectious Luciferases Escherichia coli Infections Fetus Sheep General Veterinary biology General Medicine biology.organism_classification Enterobacteriaceae Disease Models Animal Infectious Diseases Luminescent Measurements Gestation Female Bacteria Ex vivo |
| Zdroj: | Comparative Immunology, Microbiology and Infectious Diseases. 32:429-438 |
| ISSN: | 0147-9571 |
| DOI: | 10.1016/j.cimid.2008.02.001 |
| Popis: | Our objectives were to develop an ovine model for Escherichia coli -induced preterm delivery, and monitor E. coli (lux modified for photonic detection) invasion of the fetal environment—ewes (124 ± 18 d of gestation) received intrauterine inoculations using E. coli -lux as follows: control ( n = 5), 1.2 × 10 6 CFU/ml ( n = 5), 5.6 × 10 6 CFU/ml ( n = 5) E. coli -lux. Preterm delivery occurred between 48 and 120 h post-inoculation in 60%, 60% of ewes infected with 1.2, and 5.6 × 10 6 CFU/ml E. coli -lux, respectively, with presence of emitting bacteria confirmed by real-time imaging of lamb tissues. In summary, preterm delivery and/or fetal distress were observed in a majority of inoculated ewes. Finally, the use of photonic bacteria with imaging was a feasible means to monitor bacterial presence ex vivo . |
| Databáze: | OpenAIRE |
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