Study of vaginal microbiota of Holstein cows submitted to an estrus synchronization protocol with the use of intravaginal progesterone device
| Autor: | Eraldo L. Zanella, Daniel Lazzari Quadros, Giovana Ciacci Zanella, Arthur Nery da Silva, Fernanda Luiza Facioli, Carlos Bondan, Ricardo Zanella |
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| Rok vydání: | 2019 |
| Předmět: |
040301 veterinary sciences
Firmicutes media_common.quotation_subject 0403 veterinary science Andrology 03 medical and health sciences Animals Bacterial phyla Insemination Artificial Progesterone 030304 developmental biology media_common 0303 health sciences Tenericutes General Veterinary biology Microbiota Reproduction Estrus synchronization 04 agricultural and veterinary sciences biology.organism_classification Administration Intravaginal Spirochaetae Vaginal swabs Vagina Cattle Female Proteobacteria Progestins Estrus Synchronization |
| Zdroj: | Research in veterinary science. 131 |
| ISSN: | 1532-2661 |
| Popis: | The characterization of vaginal microbiota will help to understand some of the reproductive problems and mechanisms to improve cattle reproduction. The objective of this study was to characterize the vaginal microbiota of cyclical Holstein cows with different parturition orders using 16S rDNA sequencing. Animals were submitted to an estrus synchronization protocol with the use of intravaginal progesterone (P4) implants and were treated or not with ceftiofur hydrochloride. DNA samples were extracted from vaginal swabs on day 0 and 10 of the synchronization, and sequenced with the Illumina MiSeq platform with an average coverage rate of 10.000 reads per samples using a Single-End library for fragments of 300 bp. The main bacterial phyla found in the vaginal tract of Holstein cows, were Firmicutes (37.61%), Tenericutes (29.45%), Proteobacteria (17.47%) and Bacteriodetes (13.73%), followed by Actinobacteria (0.82%) and Spirochaetae (0.45%). The use of intravaginal P4 devices has increased the relative abundance of the genera Family XIII AD3011 and Family XIII unclassified (p .049). We have also observed an effect of the number of calving on the vaginal microbiota composition, showing that multiparous cows have a greater bacterial diversity than primiparous animals (p .05). The use of ceftiofur hydrochloride was effective to reduce the vaginal bacteria proliferation. This study describes for the first time the vaginal microbiota of cows synchronized with intravaginal progesterone devices, different from the traditional methods such as microbiological culture and biochemical tests. We have identified a large number of microorganisms commonly found in the gastrointestinal tract of cows, colonizing the vaginal microbiota. |
| Databáze: | OpenAIRE |
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